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Journal of Clinical Microbiology, March 2002, p. 1069-1070, Vol. 40, No. 3
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.3.1069-1070.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Comparison of Molecular and Conventional Methods for Typing of Enteroviral Isolates

Darly Joseph Manayani,1 R. V. Shaji,2 G. John Fletcher,1 Thomas Cherian,3 N. Murali,3 N. Sathish,1 Tilak Solomon,4 Chandran Gnanamuthu,4 and Gopalan Sridharan1*

Department of Clinical Virology,1 Department of Haematology,2 Department of Child Health ,3 Department of Neurology, Christian Medical College and Hospital, Vellore-632004, India4

Received 2 July 2001/ Returned for modification 17 November 2001/ Accepted 21 December 2001

Twenty-eight enteroviral isolates obtained from various clinical specimens were typed by Lim-Benyesh-Melnick (LBM) pool-based neutralization, PCR-restriction fragment length polymorphism (RFLP), and partial sequencing of the VP1 region of the enteroviral genome. Sequencing was found to be a good alternative to LBM typing, while PCR-RFLP was inadequate for identification of enteroviral isolates.

* Corresponding author. Mailing address: Department of Clinical Virology, Christian Medical College Hospital, Vellore-632 004, India. Phone: 91-416-222102, ext. 2312 or 2070. Fax: 91-416-232035. E-mail: g_sridharan{at}yahoo.com.

Journal of Clinical Microbiology, March 2002, p. 1069-1070, Vol. 40, No. 3
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.3.1069-1070.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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