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Journal of Clinical Microbiology, March 2002, p. 1091-1094, Vol. 40, No. 3
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.3.1091-1094.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

rpoB Genotypes of Mycobacterium tuberculosis Beijing Family Isolates from East Asian Countries

Lishi Qian,1 Chiyoji Abe,2 Tao-Ping Lin,3 Ming-Chih Yu,3 Sang-Nae Cho,4 Sumin Wang,5 and James T. Douglas1*

Department of Microbiology, University of Hawaii at Manoa, Honolulu, Hawaii 96822,1 Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, Kiyose-shi, Tokyo 203-8533, Japan,2 Taiwan Provincial Chronic Disease Control Bureau, 67-1, Wan-Shun-Liau, Taipei County, Taiwan,3 Department of Microbiology, Yonsei University, College of Medicine, Seoul 120-752, Korea,4 National Tuberculosis Control Center, Beijing 101149, People's Republic of China5

Received 1 October 2001/ Returned for modification 29 October 2001/ Accepted 12 December 2001

The 81-bp region of the rpoB gene in 66 Rifr Mycobacterium tuberculosis isolates from China, Japan, Korea, and Taiwan was analyzed. Twelve single-nucleotide substitutions in the rpoB gene were detected. The most prevalent mutations were at Ser-531 (52%), Asp-516 (17%), and His-526 (11%). Mutations were not found in seven (11%) of the isolates. Higher mutation rates in 50 Beijing family isolates were found than in other isolates for mutations at Asp-516 (18 and 12.5%, respectively) and His-526 (12 and 6.3%, respectively). The different rates of mutation may reflect the choice of rifamycin analogs.

* Corresponding author. Mailing address: University of Hawaii at Manoa, Department of Microbiology, 2538 The Mall (Snyder Hall 207), Honolulu, HI 96822. Phone: (808) 956-4639. Fax: (808) 956-5350. E-mail: jdouglas{at}hawaii.edu.

Journal of Clinical Microbiology, March 2002, p. 1091-1094, Vol. 40, No. 3
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.3.1091-1094.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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