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Journal of Clinical Microbiology, March 2002, p. 753-757, Vol. 40, No. 3
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.3.753-757.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Species-Level Identification of Orthopoxviruses with an Oligonucleotide Microchip

Sergey Lapa,1 Maxim Mikheev,2 Sergei Shchelkunov,2 Vladimir Mikhailovich,1* Alexander Sobolev,1 Vladimir Blinov,2 Igor Babkin,2 Alexander Guskov,2 Elena Sokunova,2 Alexander Zasedatelev,1 Lev Sandakhchiev,2 and Andrei Mirzabekov1

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow,1 State Research Center of Virology and Biotechnology "Vector," Koltsovo, Novosibirsk Region, Russia2

Received 30 April 2001/ Returned for modification 21 October 2001/ Accepted 10 December 2001

A method for species-specific detection of orthopoxviruses pathogenic for humans and animals is described. The method is based on hybridization of a fluorescently labeled amplified DNA specimen with the oligonucleotide DNA probes immobilized on a microchip (MAGIChip). The probes identify species-specific sites within the crmB gene encoding the viral analogue of tumor necrosis factor receptor, one of the most important determinants of pathogenicity in this genus of viruses. The diagnostic procedure takes 6 h and does not require any sophisticated equipment (a portable fluorescence reader can be used).

* Corresponding author. Mailing address: Biochip Technology Center, Engelhardt Institute of Molecular Biology, 32 Vavilova St., Moscow, 119991, Russia. Phone: 7 (095) 135 6529. Fax: 7 (095) 135 1405. E-mail: vmikh{at}eimb.ru.

Journal of Clinical Microbiology, March 2002, p. 753-757, Vol. 40, No. 3
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.3.753-757.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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