This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Falsey, A. R.
Right arrow Articles by Walsh, E. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Falsey, A. R.
Right arrow Articles by Walsh, E. E.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 2002, p. 817-820, Vol. 40, No. 3
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.3.817-820.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Diagnosis of Respiratory Syncytial Virus Infection: Comparison of Reverse Transcription-PCR to Viral Culture and Serology in Adults with Respiratory Illness

Ann R. Falsey,* Maria A. Formica, and Edward E. Walsh

Department of Medicine, Rochester General Hospital, University of Rochester School of Medicine and Dentistry, Rochester, New York

Received 28 September 2001/ Returned for modification 26 November 2001/ Accepted 2 January 2002

Diagnosis of respiratory syncytial virus (RSV) during acute infection in adults is difficult because of the poor sensitivity of viral culture and antigen detection. A recently developed single-tube nested reverse transcription-PCR (RT-PCR) was compared to viral culture and serology by enzyme immunoassay for the diagnosis of RSV in adults with respiratory illness. Nasal swab samples were collected during respiratory illnesses from five groups of subjects: healthy young adults, healthy elderly adults, adults with chronic heart and lung disease, nursing home residents, and adults admitted to the hospital during the winter with cardiopulmonary illnesses. Of 1,112 samples for which all three tests were available, 117 were positive by at least one method and 995 were negative by all methods. One hundred ten were considered true positives because culture and/or serology was positive. Of these, 80 (73%) were PCR positive compared to 43 (39%) that were culture positive. Seven PCR results were considered false positives due to negative culture and serology. The overall RT-PCR sensitivity was 73%, and specificity was 99%. These data indicate that RT-PCR is an excellent method for the diagnosis of acute RSV infection in adults.

* Corresponding author. Mailing address: Rochester General Hospital, 1425 Portland Ave., Rochester, NY 14621. Phone: (585) 922-4339. Fax: (585) 922-5168. E-mail: ann.falsey{at}viahealth.org.

Journal of Clinical Microbiology, March 2002, p. 817-820, Vol. 40, No. 3
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.3.817-820.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Liao, R. S., Tomalty, L. L., Majury, A., Zoutman, D. E. (2009). Comparison of Viral Isolation and Multiplex Real-Time Reverse Transcription-PCR for Confirmation of Respiratory Syncytial Virus and Influenza Virus Detection by Antigen Immunoassays. J. Clin. Microbiol. 47: 527-532 [Abstract] [Full Text]  
  • Roh, K. H., Kim, J., Nam, M.-H., Yoon, S., Lee, C. K., Lee, K., Yoo, Y., Kim, M. J., Cho, Y. (2008). Comparison of the Seeplex Reverse Transcription PCR Assay with the R-mix Viral Culture and Immunofluorescence Techniques for Detection of Eight Respiratory Viruses. Annals of Clinical & Laboratory Science 38: 41-46 [Abstract] [Full Text]  
  • Boukhvalova, M. S., Prince, G. A., Blanco, J. C. G. (2007). Respiratory Syncytial Virus Infects and Abortively Replicates in the Lungs in Spite of Preexisting Immunity. J. Virol. 81: 9443-9450 [Abstract] [Full Text]  
  • Goodrich, J. S., Miller, M. B. (2007). Comparison of Cepheid's Analyte-Specific Reagents with BD Directigen for Detection of Respiratory Syncytial Virus. J. Clin. Microbiol. 45: 604-606 [Abstract] [Full Text]  
  • Milstone, A. P., Brumble, L. M., Barnes, J., Estes, W., Loyd, J. E., Pierson, R. N. III, Dummer, S. (2006). A single-season prospective study of respiratory viral infections in lung transplant recipients. Eur Respir J 28: 131-137 [Abstract] [Full Text]  
  • Falsey, A. R., Formica, M. A., Hennessey, P. A., Criddle, M. M., Sullender, W. M., Walsh, E. E. (2006). Detection of Respiratory Syncytial Virus in Adults with Chronic Obstructive Pulmonary Disease. Am. J. Respir. Crit. Care Med. 173: 639-643 [Abstract] [Full Text]  
  • Falsey, A. R., Hennessey, P. A., Formica, M. A., Cox, C., Walsh, E. E. (2005). Respiratory Syncytial Virus Infection in Elderly and High-Risk Adults. NEJM 352: 1749-1759 [Abstract] [Full Text]  
  • Hamelin, M.-E., Boivin, G. (2005). Development and Validation of an Enzyme-Linked Immunosorbent Assay for Human Metapneumovirus Serology Based on a Recombinant Viral Protein. CVI 12: 249-253 [Abstract] [Full Text]  
  • Slinger, R., Milk, R., Gaboury, I., Diaz-Mitoma, F. (2004). Evaluation of the QuickLab RSV Test, a New Rapid Lateral-Flow Immunoassay for Detection of Respiratory Syncytial Virus Antigen. J. Clin. Microbiol. 42: 3731-3733 [Abstract] [Full Text]  
  • Templeton, K. E., Scheltinga, S. A., Beersma, M. F. C., Kroes, A. C. M., Claas, E. C. J. (2004). Rapid and Sensitive Method Using Multiplex Real-Time PCR for Diagnosis of Infections by Influenza A and Influenza B Viruses, Respiratory Syncytial Virus, and Parainfluenza Viruses 1, 2, 3, and 4. J. Clin. Microbiol. 42: 1564-1569 [Abstract] [Full Text]  
  • Mentel, R., Wegner, U., Bruns, R., Gurtler, L. (2003). Real-time PCR to improve the diagnosis of respiratory syncytial virus infection. J Med Microbiol 52: 893-896 [Abstract] [Full Text]  
  • Falsey, A. R., Formica, M. A., Treanor, J. J., Walsh, E. E. (2003). Comparison of Quantitative Reverse Transcription-PCR to Viral Culture for Assessment of Respiratory Syncytial Virus Shedding. J. Clin. Microbiol. 41: 4160-4165 [Abstract] [Full Text]  
  • van Elden, L. J. R., van Loon, A. M., van der Beek, A., Hendriksen, K. A. W., Hoepelman, A. I. M., van Kraaij, M. G. J., Schipper, P., Nijhuis, M. (2003). Applicability of a Real-Time Quantitative PCR Assay for Diagnosis of Respiratory Syncytial Virus Infection in Immunocompromised Adults. J. Clin. Microbiol. 41: 4378-4381 [Abstract] [Full Text]  
  • Cote, S., Abed, Y., Boivin, G. (2003). Comparative Evaluation of Real-Time PCR Assays for Detection of the Human Metapneumovirus. J. Clin. Microbiol. 41: 3631-3635 [Abstract] [Full Text]  
  • Rajala, M. S., Sullender, W. M., Prasad, A. K., Dar, L., Broor, S. (2003). Genetic Variability among Group A and B Respiratory Syncytial Virus Isolates from a Large Referral Hospital in New Delhi, India. J. Clin. Microbiol. 41: 2311-2316 [Abstract] [Full Text]  
  • Hu, A., Colella, M., Tam, J. S., Rappaport, R., Cheng, S.-M. (2003). Simultaneous Detection, Subgrouping, and Quantitation of Respiratory Syncytial Virus A and B by Real-Time PCR. J. Clin. Microbiol. 41: 149-154 [Abstract] [Full Text]  
  • Whiley, D. M., Syrmis, M. W., Mackay, I. M., Sloots, T. P. (2002). Detection of Human Respiratory Syncytial Virus in Respiratory Samples by LightCycler Reverse Transcriptase PCR. J. Clin. Microbiol. 40: 4418-4422 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»