This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Omoe, K.
Right arrow Articles by Shinagawa, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Omoe, K.
Right arrow Articles by Shinagawa, K.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 2002, p. 857-862, Vol. 40, No. 3
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.3.857-862.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Detection of seg, seh, and sei genes in Staphylococcus aureus Isolates and Determination of the Enterotoxin Productivities of S. aureus Isolates Harboring seg, seh, or sei Genes

Katsuhiko Omoe,1* Machiko Ishikawa,1 Yu Shimoda,1 Dong-Liang Hu,1 Shigeko Ueda,2 and Kunihiro Shinagawa1

Department of Veterinary Microbiology, Faculty of Agriculture, Iwate University, Ueda 3-18-8, Morioka, Iwate, 020-8550,1 Laboratory of Hygiene, Kagawa Nutrition University, Chiyoda, Sakado-shi, Saitama, 350-02, Japan2

Received 25 June 2001/ Returned for modification 23 October 2001/ Accepted 6 December 2001

To investigate the distribution of staphylococcal enterotoxin (SE) A to I (SEA to SEI) genes (sea to sei) in Staphylococcus aureus, 146 isolates obtained in Japan from humans involved in and samples from food poisoning outbreaks, healthy humans, cows with mastitis, and bovine raw milk were analyzed by multiplex PCR. One hundred thirteen (77.4%) S. aureus isolates were found to be positive for one or more se genes. The se genotype was classified into 14 genotypes. seg and sei coexisted in the same S. aureus strain. The newly developed sandwich enzyme-linked immunosorbent assay showed that most seh-harboring S. aureus isolates were able to produce a significant amount of SEH. However, most of the S. aureus isolates harboring seg and about 60% of the isolates harboring sei did not produce a detectable level of SEG or SEI, while reverse transcription-PCR analysis proved that the mRNAs of SEG and SEI were transcribed in S. aureus strains harboring seg and sei genes. These results suggest the importance of quantitative assessment of SEG and SEI production in foods in order to clarify the relationship between these new SEs and food poisoning.

* Corresponding author. Mailing address: Department of Veterinary Microbiology, Faculty of Agriculture, Iwate University, Ueda 3-18-8, Morioka, Iwate, 020-8550, Japan. Phone: 81-19-621-6221. Fax: 81-19-621-6223. E-mail: omo{at}iwate-u.ac.jp.

Journal of Clinical Microbiology, March 2002, p. 857-862, Vol. 40, No. 3
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.3.857-862.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Synnott, A. J., Kuang, Y., Kurimoto, M., Yamamichi, K., Iwano, H., Tanji, Y. (2009). Isolation from Sewage Influent and Characterization of Novel Staphylococcus aureus Bacteriophages with Wide Host Ranges and Potent Lytic Capabilities. Appl. Environ. Microbiol. 75: 4483-4490 [Abstract] [Full Text]  
  • Hennekinne, J.-A., Brun, V., De Buyser, M.-L., Dupuis, A., Ostyn, A., Dragacci, S. (2009). Innovative Application of Mass Spectrometry for the Characterization of Staphylococcal Enterotoxins Involved in Food Poisoning Outbreaks. Appl. Environ. Microbiol. 75: 882-884 [Abstract] [Full Text]  
  • Grumann, D., Scharf, S. S., Holtfreter, S., Kohler, C., Steil, L., Engelmann, S., Hecker, M., Volker, U., Broker, B. M. (2008). Immune Cell Activation by Enterotoxin Gene Cluster (egc)-Encoded and Non-egc Superantigens from Staphylococcus aureus. J. Immunol. 181: 5054-5061 [Abstract] [Full Text]  
  • Collery, M. M., Smyth, D. S., Twohig, J. M., Shore, A. C., Coleman, D. C., Smyth, C. J. (2008). Molecular typing of nasal carriage isolates of Staphylococcus aureus from an Irish university student population based on toxin gene PCR, agr locus types and multiple locus, variable number tandem repeat analysis. J Med Microbiol 57: 348-358 [Abstract] [Full Text]  
  • Bonness, S., Szekat, C., Novak, N., Bierbaum, G. (2008). Pulsed-Field Gel Electrophoresis of Staphylococcus aureus Isolates from Atopic Patients Revealing Presence of Similar Strains in Isolates from Children and Their Parents. J. Clin. Microbiol. 46: 456-461 [Abstract] [Full Text]  
  • Sampathkumar, P. (2007). Methicillin-Resistant Staphylococcus aureus: The Latest Health Scare. Mayo Clin Proc. 82: 1463-1467 [Full Text]  
  • Cai, Y., Kong, F., Wang, Q., Tong, Z., Sintchenko, V., Zeng, X., Gilbert, G. L. (2007). Comparison of Single- and Multilocus Sequence Typing and Toxin Gene Profiling for Characterization of Methicillin-Resistant Staphylococcus aureus. J. Clin. Microbiol. 45: 3302-3308 [Abstract] [Full Text]  
  • Pumphrey, N., Vuidepot, A., Jakobsen, B., Forsberg, G., Walse, B., Lindkvist-Petersson, K. (2007). Cutting Edge: Evidence of Direct TCR {alpha}-Chain Interaction with Superantigen. J. Immunol. 179: 2700-2704 [Abstract] [Full Text]  
  • Blaiotta, G., Fusco, V., von Eiff, C., Villani, F., Becker, K. (2006). Biotyping of Enterotoxigenic Staphylococcus aureus by Enterotoxin Gene Cluster (egc) Polymorphism and spa Typing Analyses. Appl. Environ. Microbiol. 72: 6117-6123 [Abstract] [Full Text]  
  • Fernandez, M. M., Guan, R., Swaminathan, C. P., Malchiodi, E. L., Mariuzza, R. A. (2006). Crystal Structure of Staphylococcal Enterotoxin I (SEI) in Complex with a Human Major Histocompatibility Complex Class II Molecule. J. Biol. Chem. 281: 25356-25364 [Abstract] [Full Text]  
  • Layer, F., Ghebremedhin, B., Konig, W., Konig, B. (2006). Heterogeneity of Methicillin-Susceptible Staphylococcus aureus Strains at a German University Hospital Implicates the Circulating-Strain Pool as a Potential Source of Emerging Methicillin-Resistant S. aureus Clones.. J. Clin. Microbiol. 44: 2179-2185 [Abstract] [Full Text]  
  • van Belkum, A., Melles, D. C., Snijders, S. V., van Leeuwen, W. B., Wertheim, H. F. L., Nouwen, J. L., Verbrugh, H. A., Etienne, J. (2006). Clonal Distribution and Differential Occurrence of the Enterotoxin Gene Cluster, egc, in Carriage- versus Bacteremia-Associated Isolates of Staphylococcus aureus. J. Clin. Microbiol. 44: 1555-1557 [Abstract] [Full Text]  
  • Nakayama, A., Okayama, A., Hashida, M., Yamamoto, Y., Takebe, H., Ohnaka, T., Tanaka, T., Imai, S. (2006). Development of a routine laboratory direct detection system of staphylococcal enterotoxin genes.. J Med Microbiol 55: 273-277 [Abstract] [Full Text]  
  • Naffa, R. G., Bdour, S. M., Migdadi, H. M., Shehabi, A. A. (2006). Enterotoxicity and genetic variation among clinical Staphylococcus aureus isolates in Jordan. J Med Microbiol 55: 183-187 [Abstract] [Full Text]  
  • Omoe, K., Imanishi, K., Hu, D.-L., Kato, H., Fugane, Y., Abe, Y., Hamaoka, S., Watanabe, Y., Nakane, A., Uchiyama, T., Shinagawa, K. (2005). Characterization of Novel Staphylococcal Enterotoxin-Like Toxin Type P. Infect. Immun. 73: 5540-5546 [Abstract] [Full Text]  
  • Dakic, I., Vukovic, D., Stepanovic, S., Hauschild, T., Jezek, P., Petras, P., Morrison, D. (2005). Survey of Genes Encoding Staphylococcal Enterotoxins, Toxic Shock Syndrome Toxin 1, and Exfoliative Toxins in Members of the Staphylococcus sciuri Group. J. Clin. Microbiol. 43: 4875-4876 [Abstract] [Full Text]  
  • Ikeda, T., Tamate, N., Yamaguchi, K., Makino, S.-i. (2005). Mass Outbreak of Food Poisoning Disease Caused by Small Amounts of Staphylococcal Enterotoxins A and H. Appl. Environ. Microbiol. 71: 2793-2795 [Abstract] [Full Text]  
  • Fueyo, J. M., Mendoza, M. C., Rodicio, M. R., Muniz, J., Alvarez, M. A., Martin, M. C. (2005). Cytotoxin and Pyrogenic Toxin Superantigen Gene Profiles of Staphylococcus aureus Associated with Subclinical Mastitis in Dairy Cows and Relationships with Macrorestriction Genomic Profiles. J. Clin. Microbiol. 43: 1278-1284 [Abstract] [Full Text]  
  • Hu, D.-L., Cui, J.-C., Omoe, K., Sashinami, H., Yokomizo, Y., Shinagawa, K., Nakane, A. (2005). A Mutant of Staphylococcal Enterotoxin C Devoid of Bacterial Superantigenic Activity Elicits a Th2 Immune Response for Protection against Staphylococcus aureus Infection. Infect. Immun. 73: 174-180 [Abstract] [Full Text]  
  • Lovseth, A., Loncarevic, S., Berdal, K. G. (2004). Modified Multiplex PCR Method for Detection of Pyrogenic Exotoxin Genes in Staphylococcal Isolates. J. Clin. Microbiol. 42: 3869-3872 [Abstract] [Full Text]  
  • Holtfreter, S., Bauer, K., Thomas, D., Feig, C., Lorenz, V., Roschack, K., Friebe, E., Selleng, K., Lovenich, S., Greve, T., Greinacher, A., Panzig, B., Engelmann, S., Lina, G., Broker, B. M. (2004). egc-Encoded Superantigens from Staphylococcus aureus Are Neutralized by Human Sera Much Less Efficiently than Are Classical Staphylococcal Enterotoxins or Toxic Shock Syndrome Toxin. Infect. Immun. 72: 4061-4071 [Abstract] [Full Text]  
  • Omoe, K., Imanishi, K., Hu, D.-L., Kato, H., Takahashi-Omoe, H., Nakane, A., Uchiyama, T., Shinagawa, K. (2004). Biological Properties of Staphylococcal Enterotoxin-Like Toxin Type R. Infect. Immun. 72: 3664-3667 [Abstract] [Full Text]  
  • Sergeev, N., Volokhov, D., Chizhikov, V., Rasooly, A. (2004). Simultaneous Analysis of Multiple Staphylococcal Enterotoxin Genes by an Oligonucleotide Microarray Assay. J. Clin. Microbiol. 42: 2134-2143 [Abstract] [Full Text]  
  • Omoe, K., Hu, D.-L., Takahashi-Omoe, H., Nakane, A., Shinagawa, K. (2003). Identification and Characterization of a New Staphylococcal Enterotoxin-Related Putative Toxin Encoded by Two Kinds of Plasmids. Infect. Immun. 71: 6088-6094 [Abstract] [Full Text]  
  • Klotz, M., Opper, S., Heeg, K., Zimmermann, S. (2003). Detection of Staphylococcus aureus Enterotoxins A to D by Real-Time Fluorescence PCR Assay. J. Clin. Microbiol. 41: 4683-4687 [Abstract] [Full Text]  
  • Hu, D.-L., Omoe, K., Shimoda, Y., Nakane, A., Shinagawa, K. (2003). Induction of Emetic Response to Staphylococcal Enterotoxins in the House Musk Shrew (Suncus murinus). Infect. Immun. 71: 567-570 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»