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Journal of Clinical Microbiology, March 2002, p. 937-942, Vol. 40, No. 3
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.3.937-942.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Identification and Molecular Characterization of a Bovine G3 Rotavirus Which Causes Age-Independent Diarrhea in Cattle

L. El-Attar,¹ W. Dhaliwal,^1, M. Iturriza-Gómara,² and J. C. Bridger^1*

Department of Pathology and Infectious Diseases, The Royal Veterinary College, London NW1 0TU,¹ Clinical Microbiology and Public Health Laboratory, Addenbrooke's Hospital, Cambridge CB2 2QW, United Kingdom²

Received 13 August 2001/ Returned for modification 18 November 2001/ Accepted 16 December 2001

G3 rotaviruses have been reported rarely in cattle, and none have been characterized. We report the first genomic characterization of a bovine G3 rotavirus, CP-1, which had been biologically characterized in vivo and shown to cause age-independent diarrhea. CP-1 was a G3 rotavirus as its VP7 had 92 to 96% deduced amino acid identity to those of G3 rotaviruses. However, initially, CP-1 was identified as a G10 rotavirus by RT-PCR even though the CP-1 VP7 had only 81 to 85% deduced amino acid identity to those of G10 rotaviruses. Rotavirus CP-1 was of P[5] specificity, a type common in cattle, and had a bovine NSP1 and NSP4. These results added another animal species to those in which G3 rotaviruses have been found, characterized a bovine rotavirus which caused age-independent diarrhea in calves, and raised the possibility that bovine G3 rotaviruses may be misdiagnosed as G10 rotaviruses.

Present address: Digestive Diseases Research Centre, St. Bartholomew's and the Royal London School of Medicine and Dentistry, London E1 2AE, United Kingdom.

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