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Journal of Clinical Microbiology, April 2002, p. 1237-1243, Vol. 40, No. 4
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.4.1237-1243.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Epidemiology of Extended-Spectrum ß-Lactamase-Producing Enterobacter Isolates in a Spanish Hospital during a 12-Year Period

Rafael Cantón,* Antonio Oliver, Teresa M. Coque, María del Carmen Varela, José Claudio Pérez-Díaz, and Fernando Baquero

Servicio de Microbiología Hospital Ramón y Cajal, Madrid, Spain

Received 10 October 2001/ Returned for modification 13 December 2001/ Accepted 24 January 2002

Fifteen Enterobacter clinical isolates (11 Enterobacter cloacae isolates, 3 Enterobacter aerogenes isolates, and 1 Enterobacter gergoviae isolate), representing 0.4% of all Enterobacter isolates recovered in our hospital from 1989 to 2000, were suspected of harboring an extended-spectrum ß-lactamase (ESBL). These isolates were recovered from 14 different patients. ESBLs were transferred by conjugation into an Escherichia coli recipient strain. Pulsed-field gel electrophoresis (PFGE) revealed a single clone of E. aerogenes and six different clones of E. cloacae. Four of these E. cloacae clonal types were represented by only one isolate each, but the other two were represented by three and four isolates, respectively. Isoelectric focusing, susceptibility phenotyping, PCR analysis, and sequencing demonstrated the presence of three different ESBLs. The most frequent was the recently characterized CTX-M-10 ESBL, which was found in the E. gergoviae isolate and in all but one of the E. cloacae isolates. The remaining E. cloacae isolate harbored a TEM-27 ESBL, and the three E. aerogenes isolates harbored a TEM-24 ESBL. PFGE revealed that our E. aerogenes strain was indistinguishable from the French TEM-24-producing E. aerogenes endemic clone. Although a low prevalence of ESBL-producing Enterobacter isolates was found in our institution over a 12-year period, a diversity of nonepidemic E. cloacae clones was detected, as was the persistence of the CTX-M-10 ß-lactamase. The presence of the TEM-24-producing E. aerogenes French clone in our institution also demonstrates the intercountry dissemination of ESBL-producing isolates.

* Corresponding author. Mailing address: Servicio de Microbiología, Hospital Ramón y Cajal, 28034-Madrid, Spain. Phone: 34 91 336 8330. Fax: 34 91 336 8809. E-mail: rcanton{at}hrc.insalud.es.

Journal of Clinical Microbiology, April 2002, p. 1237-1243, Vol. 40, No. 4
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.4.1237-1243.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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