This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Warren, R. M.
Right arrow Articles by van Helden, P. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Warren, R. M.
Right arrow Articles by van Helden, P. D.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 2002, p. 1277-1282, Vol. 40, No. 4
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.4.1277-1282.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Evolution of the IS6110-Based Restriction Fragment Length Polymorphism Pattern during the Transmission of Mycobacterium tuberculosis

R. M. Warren,1 G. D. van der Spuy,1 M. Richardson,1 N. Beyers,2 C. Booysen,2 M. A. Behr,3 and P. D. van Helden1*

MRC Centre for Molecular and Cellular Biology, Department of Medical Biochemistry,1 Department of Paediatrics and Child Health, Faculty of Health Sciences, University of Stellenbosch, Tygerberg 7505, South Africa ,2 Department of Medicine, McGill University Health Centre, Montreal H3G 1A4, Canada3

Received 25 October 2001/ Returned for modification 4 December 2001/ Accepted 21 January 2002

Interpretation of the molecular epidemiological data of Mycobacterium tuberculosis is dependent on the validity of the assumptions that have been made. It is assumed that the IS6110 banding pattern is sufficiently stable to define epidemiological events representing ongoing transmission. However, molecular epidemiological data also support the observation that the IS6110 banding pattern may change over time. Factors affecting this rate may include the nature and duration of disease in a host and the opportunity to experience different host environments during the transmission cycle. To estimate the rate of IS6110 change occurring during the process of transmission, M. tuberculosis isolates from epidemiologically linked patients were genotypically characterized by restriction fragment length polymorphism (RFLP) analysis. The identification of IS6110 banding pattern changes during ongoing transmission suggested that a rate could be estimated. IS6110 change was significantly associated with strains with >5 IS6110 elements (P = 0.013) and was not observed in low-copy-number isolates. The minimum rate of appearance of variant strains was calculated to be 0.14 variant cases per source-case per year. This data suggest that clustering of isolates based on identical RFLP patterns is expected to underestimate transmission in patients infected with high-copy-number isolates. A model based on the rate of appearance of both variant and invariant strains demonstrates that the genotypically defined population structure may change by 18.6% during the study period of approximately 6.5 years. The implications for the use of RFLP data for epidemiologic study are discussed.

* Corresponding author. Mailing address: MRC Centre for Molecular and Cellular Biology, Department of Medical Biochemistry, University of Stellenbosch, P.O. Box 19063, Tygerberg 7505, South Africa. Phone: 27-21-9389401. Fax: 27-21-9317810 or 9389476. E-mail: PVH{at}SUN.AC.ZA.

Journal of Clinical Microbiology, April 2002, p. 1277-1282, Vol. 40, No. 4
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.4.1277-1282.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hanekom, M., van der Spuy, G. D., van Pittius, N. C. G., McEvoy, C. R. E., Hoek, K. G. P., Ndabambi, S. L., Jordaan, A. M., Victor, T. C., van Helden, P. D., Warren, R. M. (2008). Discordance between Mycobacterial Interspersed Repetitive-Unit-Variable-Number Tandem-Repeat Typing and IS6110 Restriction Fragment Length Polymorphism Genotyping for Analysis of Mycobacterium tuberculosis Beijing Strains in a Setting of High Incidence of Tuberculosis . J. Clin. Microbiol. 46: 3338-3345 [Abstract] [Full Text]  
  • Durmaz, R., Zozio, T., Gunal, S., Allix, C., Fauville-Dufaux, M., Rastogi, N. (2007). Population-Based Molecular Epidemiological Study of Tuberculosis in Malatya, Turkey. J. Clin. Microbiol. 45: 4027-4035 [Abstract] [Full Text]  
  • Wong, K. C., Leong, W. M., Law, H. K. W., Ip, K. F., Lam, J. T. H., Yuen, K. Y., Ho, P. L., Tse, W. S., Weng, X. H., Zhang, W. H., Chen, S., Yam, W. C. (2007). Molecular Characterization of Clinical Isolates of Mycobacterium tuberculosis and Their Association with Phenotypic Virulence in Human Macrophages. CVI 14: 1279-1284 [Abstract] [Full Text]  
  • Oelemann, M. C., Diel, R., Vatin, V., Haas, W., Rusch-Gerdes, S., Locht, C., Niemann, S., Supply, P. (2007). Assessment of an Optimized Mycobacterial Interspersed Repetitive- Unit-Variable-Number Tandem-Repeat Typing System Combined with Spoligotyping for Population-Based Molecular Epidemiology Studies of Tuberculosis. J. Clin. Microbiol. 45: 691-697 [Abstract] [Full Text]  
  • Ignatova, A., Dubiley, S., Stepanshina, V., Shemyakin, I. (2006). Predominance of multi-drug-resistant LAM and Beijing family strains among Mycobacterium tuberculosis isolates recovered from prison inmates in Tula Region, Russia.. J Med Microbiol 55: 1413-1418 [Abstract] [Full Text]  
  • Gopaul, K. K., Brown, T. J., Gibson, A. L., Yates, M. D., Drobniewski, F. A. (2006). Progression Toward an Improved DNA Amplification-Based Typing Technique in the Study of Mycobacterium tuberculosis Epidemiology.. J. Clin. Microbiol. 44: 2492-2498 [Abstract] [Full Text]  
  • Cave, M. D., Yang, Z. H., Stefanova, R., Fomukong, N., Ijaz, K., Bates, J., Eisenach, K. D. (2005). Epidemiologic Import of Tuberculosis Cases Whose Isolates Have Similar but Not Identical IS6110 Restriction Fragment Length Polymorphism Patterns. J. Clin. Microbiol. 43: 1228-1233 [Abstract] [Full Text]  
  • Shamputa, I. C., Rigouts, L., Eyongeta, L. A., El Aila, N. A., van Deun, A., Salim, A. H., Willery, E., Locht, C., Supply, P., Portaels, F. (2004). Genotypic and Phenotypic Heterogeneity among Mycobacterium tuberculosis Isolates from Pulmonary Tuberculosis Patients. J. Clin. Microbiol. 42: 5528-5536 [Abstract] [Full Text]  
  • Blackwood, K. S., Wolfe, J. N., Kabani, A. M. (2004). Application of Mycobacterial Interspersed Repetitive Unit Typing to Manitoba Tuberculosis Cases: Can Restriction Fragment Length Polymorphism Be Forgotten?. J. Clin. Microbiol. 42: 5001-5006 [Abstract] [Full Text]  
  • Sun, Y.-J., Lee, A. S. G., Ng, S. T., Ravindran, S., Kremer, K., Bellamy, R., Wong, S.-Y., van Soolingen, D., Supply, P., Paton, N. I. (2004). Characterization of Ancestral Mycobacterium tuberculosis by Multiple Genetic Markers and Proposal of Genotyping Strategy. J. Clin. Microbiol. 42: 5058-5064 [Abstract] [Full Text]  
  • Eilers, P. H. C., van Soolingen, D., Thi Ngoc Lan, N., Warren, R. M., Borgdorff, M. W. (2004). Transposition Rates of Mycobacterium tuberculosis IS6110 Restriction Fragment Length Polymorphism Patterns. J. Clin. Microbiol. 42: 2461-2464 [Abstract] [Full Text]  
  • Rajakumar, K., Shafi, J., Smith, R. J., Stabler, R. A., Andrew, P. W., Modha, D., Bryant, G., Monk, P., Hinds, J., Butcher, P. D., Barer, M. R. (2004). Use of Genome Level-Informed PCR as a New Investigational Approach for Analysis of Outbreak-Associated Mycobacterium tuberculosis Isolates. J. Clin. Microbiol. 42: 1890-1896 [Abstract] [Full Text]  
  • Verver, S., Warren, R. M, Munch, Z., Vynnycky, E., van Helden, P. D, Richardson, M., van der Spuy, G. D, Enarson, D. A, Borgdorff, M. W, Behr, M. A, Beyers, N. (2004). Transmission of tuberculosis in a high incidence urban community in South Africa. Int J Epidemiol 33: 351-357 [Abstract] [Full Text]  
  • Richardson, M., van der Spuy, G. D., Sampson, S. L., Beyers, N., van Helden, P. D., Warren, R. M. (2004). Stability of Polymorphic GC-Rich Repeat Sequence-Containing Regions of Mycobacterium tuberculosis. J. Clin. Microbiol. 42: 1302-1304 [Abstract] [Full Text]  
  • van der Spuy, G. D., Warren, R. M., Richardson, M., Beyers, N., Behr, M. A., van Helden, P. D. (2003). Use of Genetic Distance as a Measure of Ongoing Transmission of Mycobacterium tuberculosis. J. Clin. Microbiol. 41: 5640-5644 [Abstract] [Full Text]  
  • Nguyen, D., Proulx, J.-F., Westley, J., Thibert, L., Dery, S., Behr, M. A. (2003). Tuberculosis in the Inuit Community of Quebec, Canada. Am. J. Respir. Crit. Care Med. 168: 1353-1357 [Abstract] [Full Text]  
  • Savine, E., Warren, R. M., van der Spuy, G. D., Beyers, N., van Helden, P. D., Locht, C., Supply, P. (2002). Stability of Variable-Number Tandem Repeats of Mycobacterial Interspersed Repetitive Units from 12 Loci in Serial Isolates of Mycobacterium tuberculosis. J. Clin. Microbiol. 40: 4561-4566 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»