This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nosek, J. Articles by Fukuhara, H. Search for Related Content PubMed PubMed Citation Articles by Nosek, J. Articles by Fukuhara, H.

Mitochondrial Telomeres as Molecular Markers for Identification of the Opportunistic Yeast Pathogen Candida parapsilosis

Jozef Nosek,^1* L'ubomír Tomáka,² Adriana Ryovská,¹ and Hiroshi Fukuhara³

Departments of Biochemistry,¹ Genetics, Faculty of Natural Sciences, Comenius University, 84215 Bratislava, Slovakia,² Institut Curie, Section de Recherche, Centre Universitaire Paris XI, 91405 Orsay, France³

Received 25 July 2001/ Returned for modification 14 December 2001/ Accepted 24 January 2002

Recent studies have demonstrated that a large number of organisms carry linear mitochondrial DNA molecules possessing specialized telomeric structures at their ends. Based on this specific structural feature of linear mitochondrial genomes, we have developed an approach for identification of the opportunistic yeast pathogen Candida parapsilosis. The strategy for identification of C. parapsilosis strains is based on PCR amplification of specific DNA sequences derived from the mitochondrial telomere region. This assay is complemented by immunodetection of a protein component of mitochondrial telomeres. The results demonstrate that mitochondrial telomeres represent specific molecular markers with potential applications in yeast diagnostics and taxonomy.

This article has been cited by other articles:

• Kocsube, S., Toth, M., Vagvolgyi, C., Doczi, I., Pesti, M., Pocsi, I., Szabo, J., Varga, J. (2007). Occurrence and genetic variability of Candida parapsilosis sensu lato in Hungary. J Med Microbiol 56: 190-195 [Abstract] [Full Text]  
• Lasker, B. A., Butler, G., Lott, T. J. (2006). Molecular Genotyping of Candida parapsilosis Group I Clinical Isolates by Analysis of Polymorphic Microsatellite Markers.. J. Clin. Microbiol. 44: 750-759 [Abstract] [Full Text]  
• Tavanti, A., Davidson, A. D., Gow, N. A. R., Maiden, M. C. J., Odds, F. C. (2005). Candida orthopsilosis and Candida metapsilosis spp. nov. To Replace Candida parapsilosis Groups II and III. J. Clin. Microbiol. 43: 284-292 [Abstract] [Full Text]  
• Rycovska, A., Valach, M., Tomaska, L., Bolotin-Fukuhara, M., Nosek, J. (2004). Linear versus circular mitochondrial genomes: intraspecies variability of mitochondrial genome architecture in Candida parapsilosis. Microbiology 150: 1571-1580 [Abstract] [Full Text]