This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bougnoux, M.-E.
Right arrow Articles by d'Enfert, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bougnoux, M.-E.
Right arrow Articles by d'Enfert, C.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 2002, p. 1290-1297, Vol. 40, No. 4
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.4.1290-1297.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Usefulness of Multilocus Sequence Typing for Characterization of Clinical Isolates of Candida albicans

M.-E. Bougnoux,1,2* S. Morand,3 and C. d'Enfert1

Unité Microbiologie et Environnement, Institut Pasteur, Paris,1 Laboratoire de Parasitologie-Mycologie, Service de Microbiologie, Hôpital Ambroise-Paré, Université Paris V, Boulogne-Billancourt,2 Centre d'Ecologie et de Biologie Tropicale et Méditerranéenne, UMR5555, CNRS, Université de Perpignan, Perpignan, France3

Received 24 October 2001/ Returned for modification 18 December 2001/ Accepted 20 January 2002

Molecular characterization of Candida albicans isolates is essential for understanding the epidemiology of nosocomial infections caused by this yeast. Here, we investigated the potential value of multilocus sequence typing (MLST) for characterizing epidemiologically related or unrelated C. albicans strains of various clinical origins. Accordingly, we sequenced the internal regions (loci) of six selected housekeeping genes of 40 C. albicans clinical isolates and 2 reference strains. In all, 68 polymorphic nucleotide sites were identified, of which 65 were found to be heterozygous in at least one isolate. Ten to 24 different genotypes were observed at the different loci, resulting, when combined, in 39 unique genotype combinations or diploid sequence types (DSTs). When MLST was applied to 26 epidemiologically unrelated isolates and the 2 reference strains, it allowed the identification of 27 independent DSTs, thus demonstrating a discriminatory power of 99.7. Using multidimensional scaling together with the minimum spanning tree method to analyze interstrain relationships, we identified six groups of genetically related isolates on the basis of bootstrap values of greater than 900. Application of MLST to 14 epidemiologically related isolates showed that those recovered from patients in the same hospital ward during the same 3 months had specific DSTs, although 73% of these isolates were genetically very close. This suggests that MLST can trace minute variations in the sequences of related isolates. Overall, MLST proved to be a highly discriminatory and stable method for unambiguous characterization of C. albicans.

* Corresponding author. Mailing address: Laboratoire de Parasitologie-Mycologie, Service de Microbiologie, Hôpital Ambroise-Paré, 9, Avenue du Général de Gaulle, 92100 Boulogne-Billancourt, France. Phone: 33 1 49 09 55 45. Fax: 33 1 49 09 59 21. E-mail: marie-elisabeth.bougnoux{at}apr.ap-hop-paris.fr.

Journal of Clinical Microbiology, April 2002, p. 1290-1297, Vol. 40, No. 4
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.4.1290-1297.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • van Hal, S. J., Gilgado, F., Doyle, T., Barratt, J., Stark, D., Meyer, W., Harkness, J. (2009). Clinical Significance and Phylogenetic Relationship of Novel Australian Pneumocystis jirovecii Genotypes. J. Clin. Microbiol. 47: 1818-1823 [Abstract] [Full Text]  
  • Odds, F. C., Jacobsen, M. D. (2008). Multilocus Sequence Typing of Pathogenic Candida Species. Eukaryot Cell 7: 1075-1084 [Full Text]  
  • McManus, B. A., Coleman, D. C., Moran, G., Pinjon, E., Diogo, D., Bougnoux, M.-E., Borecka-Melkusova, S., Bujdakova, H., Murphy, P., d'Enfert, C., Sullivan, D. J. (2008). Multilocus Sequence Typing Reveals that the Population Structure of Candida dubliniensis Is Significantly Less Divergent than That of Candida albicans. J. Clin. Microbiol. 46: 652-664 [Abstract] [Full Text]  
  • Woo, P. C. Y., Lau, C. C. Y., Chong, K. T. K., Tse, H., Tsang, D. N. C., Lee, R. A., Tse, C. W. S., Que, T.-l., Chung, L. M. W., Ngan, A. H. Y., Hui, W.-t., Wong, S. S. Y., Lau, S. K. P., Yuen, K.-y. (2007). MP1 Homologue-Based Multilocus Sequence System for Typing the Pathogenic Fungus Penicillium marneffei: a Novel Approach Using Lineage-Specific Genes. J. Clin. Microbiol. 45: 3647-3654 [Abstract] [Full Text]  
  • Lin, C.-Y., Chen, Y.-C., Lo, H.-J., Chen, K.-W., Li, S.-Y. (2007). Assessment of Candida glabrata Strain Relatedness by Pulsed-Field Gel Electrophoresis and Multilocus Sequence Typing. J. Clin. Microbiol. 45: 2452-2459 [Abstract] [Full Text]  
  • Balajee, S. A., Tay, S. T., Lasker, B. A., Hurst, S. F., Rooney, A. P. (2007). Characterization of a Novel Gene for Strain Typing Reveals Substructuring of Aspergillus fumigatus across North America. Eukaryot Cell 6: 1392-1399 [Abstract] [Full Text]  
  • Wise, M. G., Healy, M., Reece, K., Smith, R., Walton, D., Dutch, W., Renwick, A., Huong, J., Young, S., Tarrand, J., Kontoyiannis, D. P. (2007). Species identification and strain differentiation of clinical Candida isolates using the DiversiLab system of automated repetitive sequence-based PCR. J Med Microbiol 56: 778-787 [Abstract] [Full Text]  
  • Odds, F. C., Bougnoux, M.-E., Shaw, D. J., Bain, J. M., Davidson, A. D., Diogo, D., Jacobsen, M. D., Lecomte, M., Li, S.-Y., Tavanti, A., Maiden, M. C. J., Gow, N. A. R., d'Enfert, C. (2007). Molecular Phylogenetics of Candida albicans. Eukaryot Cell 6: 1041-1052 [Abstract] [Full Text]  
  • Odds, F. C., Davidson, A. D., Jacobsen, M. D., Tavanti, A., Whyte, J. A., Kibbler, C. C., Ellis, D. H., Maiden, M. C. J., Shaw, D. J., Gow, N. A. R. (2006). Candida albicans Strain Maintenance, Replacement, and Microvariation Demonstrated by Multilocus Sequence Typing.. J. Clin. Microbiol. 44: 3647-3658 [Abstract] [Full Text]  
  • Lasker, B. A. (2006). Nucleotide Sequence-Based Analysis for Determining the Molecular Epidemiology of Penicillium marneffei.. J. Clin. Microbiol. 44: 3145-3153 [Abstract] [Full Text]  
  • Chen, K.-W., Chen, Y.-C., Lo, H.-J., Odds, F. C., Wang, T.-H., Lin, C.-Y., Li, S.-Y. (2006). Multilocus Sequence Typing for Analyses of Clonality of Candida albicans Strains in Taiwan.. J. Clin. Microbiol. 44: 2172-2178 [Abstract] [Full Text]  
  • Bougnoux, M.-E., Diogo, D., Francois, N., Sendid, B., Veirmeire, S., Colombel, J. F., Bouchier, C., Van Kruiningen, H., d'Enfert, C., Poulain, D. (2006). Multilocus Sequence Typing Reveals Intrafamilial Transmission and Microevolutions of Candida albicans Isolates from the Human Digestive Tract.. J. Clin. Microbiol. 44: 1810-1820 [Abstract] [Full Text]  
  • Laverdiere, M., Lalonde, R. G., Baril, J.-G., Sheppard, D. C., Park, S., Perlin, D. S. (2006). Progressive loss of echinocandin activity following prolonged use for treatment of Candida albicans oesophagitis. J Antimicrob Chemother 57: 705-708 [Abstract] [Full Text]  
  • Nebavi, F., Ayala, F. J., Renaud, F., Bertout, S., Eholie, S., Moussa, K., Mallie, M., de Meeus, T. (2006). Clonal population structure and genetic diversity of Candida albicans in AIDS patients from Abidjan (Cote d'Ivoire). Proc. Natl. Acad. Sci. USA 103: 3663-3668 [Abstract] [Full Text]  
  • Vanittanakom, N., Cooper, C. R. Jr., Fisher, M. C., Sirisanthana, T. (2006). Penicillium marneffei Infection and Recent Advances in the Epidemiology and Molecular Biology Aspects. Clin. Microbiol. Rev. 19: 95-110 [Abstract] [Full Text]  
  • Viviani, M. A., Cogliati, M., Esposto, M. C., Prigitano, A., Tortorano, A. M. (2006). Four-Year Persistence of a Single Candida albicans Genotype Causing Bloodstream Infections in a Surgical Ward Proven by Multilocus Sequence Typing. J. Clin. Microbiol. 44: 218-221 [Abstract] [Full Text]  
  • Tavanti, A., Davidson, A. D., Johnson, E. M., Maiden, M. C. J., Shaw, D. J., Gow, N. A. R., Odds, F. C. (2005). Multilocus Sequence Typing for Differentiation of Strains of Candida tropicalis. J. Clin. Microbiol. 43: 5593-5600 [Abstract] [Full Text]  
  • Tavanti, A., Davidson, A. D., Fordyce, M. J., Gow, N. A. R., Maiden, M. C. J., Odds, F. C. (2005). Population Structure and Properties of Candida albicans, as Determined by Multilocus Sequence Typing. J. Clin. Microbiol. 43: 5601-5613 [Abstract] [Full Text]  
  • Sampaio, P., Gusmao, L., Correia, A., Alves, C., Rodrigues, A. G., Pina-Vaz, C., Amorim, A., Pais, C. (2005). New Microsatellite Multiplex PCR for Candida albicans Strain Typing Reveals Microevolutionary Changes. J. Clin. Microbiol. 43: 3869-3876 [Abstract] [Full Text]  
  • Tavanti, A., Davidson, A. D., Gow, N. A. R., Maiden, M. C. J., Odds, F. C. (2005). Candida orthopsilosis and Candida metapsilosis spp. nov. To Replace Candida parapsilosis Groups II and III. J. Clin. Microbiol. 43: 284-292 [Abstract] [Full Text]  
  • Healy, M., Reece, K., Walton, D., Huong, J., Shah, K., Kontoyiannis, D. P. (2004). Identification to the Species Level and Differentiation between Strains of Aspergillus Clinical Isolates by Automated Repetitive-Sequence-Based PCR. J. Clin. Microbiol. 42: 4016-4024 [Abstract] [Full Text]  
  • Robles, J. C., Koreen, L., Park, S., Perlin, D. S. (2004). Multilocus Sequence Typing Is a Reliable Alternative Method to DNA Fingerprinting for Discriminating among Strains of Candida albicans. J. Clin. Microbiol. 42: 2480-2488 [Abstract] [Full Text]  
  • Forche, A., Magee, P. T., Magee, B. B., May, G. (2004). Genome-Wide Single-Nucleotide Polymorphism Map for Candida albicans. Eukaryot Cell 3: 705-714 [Abstract] [Full Text]  
  • Feil, E. J., Li, B. C., Aanensen, D. M., Hanage, W. P., Spratt, B. G. (2004). eBURST: Inferring Patterns of Evolutionary Descent among Clusters of Related Bacterial Genotypes from Multilocus Sequence Typing Data. J. Bacteriol. 186: 1518-1530 [Abstract] [Full Text]  
  • Dodgson, A. R., Pujol, C., Denning, D. W., Soll, D. R., Fox, A. J. (2003). Multilocus Sequence Typing of Candida glabrata Reveals Geographically Enriched Clades. J. Clin. Microbiol. 41: 5709-5717 [Abstract] [Full Text]  
  • Bougnoux, M.-E., Tavanti, A., Bouchier, C., Gow, N. A. R., Magnier, A., Davidson, A. D., Maiden, M. C. J., d'Enfert, C., Odds, F. C. (2003). Collaborative Consensus for Optimized Multilocus Sequence Typing of Candida albicans. J. Clin. Microbiol. 41: 5265-5266 [Abstract] [Full Text]  
  • Wang, X.-M., Noble, L., Kreiswirth, B. N., Eisner, W., McClements, W., Jansen, K. U., Anderson, A. S. (2003). Evaluation of a multilocus sequence typing system for Staphylococcus epidermidis. J Med Microbiol 52: 989-998 [Abstract] [Full Text]  
  • Sendid, B., Caillot, D., Baccouch-Humbert, B., Klingspor, L., Grandjean, M., Bonnin, A., Poulain, D. (2003). Contribution of the Platelia Candida-Specific Antibody and Antigen Tests to Early Diagnosis of Systemic Candida tropicalis Infection in Neutropenic Adults. J. Clin. Microbiol. 41: 4551-4558 [Abstract] [Full Text]  
  • Zhang, N., Harrex, A. L., Holland, B. R., Fenton, L. E., Cannon, R. D., Schmid, J. (2003). Sixty Alleles of the ALS7 Open Reading Frame in Candida albicans: ALS7 Is a Hypermutable Contingency Locus. Genome Res 13: 2005-2017 [Abstract] [Full Text]  
  • Tavanti, A., Gow, N. A. R., Senesi, S., Maiden, M. C. J., Odds, F. C. (2003). Optimization and Validation of Multilocus Sequence Typing for Candida albicans. J. Clin. Microbiol. 41: 3765-3776 [Abstract] [Full Text]  
  • van Leeuwen, W. B., Jay, C., Snijders, S., Durin, N., Lacroix, B., Verbrugh, H. A., Enright, M. C., Troesch, A., van Belkum, A. (2003). Multilocus Sequence Typing of Staphylococcus aureus with DNA Array Technology. J. Clin. Microbiol. 41: 3323-3326 [Abstract] [Full Text]  
  • Matsuda, M., Kaneko, A., Stanley, T., Millar, B. C., Miyajima, M., Murphy, P. G., Moore, J. E. (2003). Characterization of Urease-Positive Thermophilic Campylobacter Subspecies by Multilocus Enzyme Electrophoresis Typing. Appl. Environ. Microbiol. 69: 3308-3310 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»