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Journal of Clinical Microbiology, April 2002, p. 1400-1405, Vol. 40, No. 4
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.4.1400-1405.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Immunoglobulin M (IgM)-Glycoinositolphospholipid Enzyme-Linked Immunosorbent Assay: an Immunoenzymatic Assay for Discrimination between Patients with Acute Toxoplasmosis and Those with Persistent Parasite-Specific IgM Antibodies

Mónica Giraldo,1,2 Ricardo W. D. Portela,1,2 Mirian Snege,3 Paulo G. Leser,3 Mário E. Camargo,3 José Roberto Mineo,4 and Ricardo T. Gazzinelli1,2*

Department of Biochemistry and Immunology—UFMG, 31270-901 Belo Horizonte,1 Laboratory of Immunopathology, CPqRR—FIOCRUZ, 30190-002 Belo Horizonte,2 Department of Immunology, Microbiology and Parasitology—UFU, 38400-902 Uberlândia, MG,3 Fleury Laboratory, 01333-910 São Paulo, SP, Brazil4

Received 15 October 2001/ Returned for modification 5 December 2001/ Accepted 27 January 2002

In the present study we developed an enzyme-linked immunosorbent assay (ELISA) to measure immunoglobulin M (IgM) specific for glycoinositolphospholipids (GIPL) derived from tachyzoite membrane (IgM-GIPL ELISA). The sensitivity and specificity of the assay were compared with those of commercially available Toxoplasma-specific IgM serological tests, namely, immunofluorescence assay (IFA) with fixed tachyzoites and capture ELISA employing tachyzoite extracts. Our results show that all patients with acute toxoplasmosis, as determined by clinical data and conventional serological tests, were also positive by the IgM-GIPL ELISA. Interestingly, many patients that were classified as indeterminate, who had IgG with high avidity but positive results in the IgM-specific IFA and capture ELISA, were negative by the IgM-GIPL ELISA. Finally, we tested the sera from patients with rheumatoid arthritis and various parasitic infections and found no evidence of false positives in the IgM-GIPL ELISA.

* Corresponding author. Mailing address: Laboratory of Immunopathology, Centro de Pesquisas René Rachou, FIOCRUZ, Av. Augusto de Lima 1715, 30190-002, Belo Horizonte, MG, Brazil. Phone: 0055 31 3295 3566. Fax: 0055 31 3295 3115. E-mail: ritoga{at}dedalus.lcc.ufmg.br.

Journal of Clinical Microbiology, April 2002, p. 1400-1405, Vol. 40, No. 4
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.4.1400-1405.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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