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Journal of Clinical Microbiology, April 2002, p. 1451-1455, Vol. 40, No. 4
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.4.1451-1455.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Quantitative Detection of Mycoplasma genitalium from First-Pass Urine of Men with Urethritis and Asymptomatic Men by Real-Time PCR

Takashi Yoshida,1 Takashi Deguchi,2* Masayasu Ito,2 Shin-Ichi Maeda,3 Masayoshi Tamaki,3 and Hiroaki Ishiko1

Research and Development Department, Mitsubishi Kagaku Bio-Clinical Laboratories, Inc., Tokyo 174-8555,1 Department of Urology, Gifu University School of Medicine, Gifu 500-8705,2 Department of Urology, Toyota Memorial Hospital, Toyota 471-8513, Japan3

Received 24 September 2001/ Returned for modification 17 December 2001/ Accepted 2 January 2002

We developed a TaqMan-based real-time PCR assay for quantifying Mycoplasma genitalium. This assay is able to specifically quantify concentrations of the M. genitalium 16S rRNA gene ranging from 107 to 10 copies/reaction. Using the TaqMan assay, we quantified the M. genitalium 16S rRNA gene in first-pass urine of men with urethritis and asymptomatic men who were positive for M. genitalium by PCR- and phylogeny-based assay. Of 130 men with gonococcal urethritis (GU), five were positive for M. genitalium. The mycoplasma load for each specimen was <5 x 10 copies/ml. Of 84 men with chlamydial non-GU (CNGU), seven were positive for M. genitalium. One man had an M. genitalium load of <5 x 10 copies/ml, and six men had loads ranging from 1.1 x 107 to 2.7 x 102 copies/ml. Of 86 men with nonchlamydial NGU (NCNGU), 17 were positive for M. genitalium. The mycoplasma loads for these men ranged from 3.3 x 106 to 2.3 x 102 copies/ml. Of 76 asymptomatic men, only two were positive for M. genitalium. For these men, the loads were 2 x 102 and <5 x 10 copies/ml. The patients with NGU had significantly higher concentrations of M. genitalium in their first-pass urine than did men with GU (P < 0.01) or asymptomatic men (P < 0.05). In addition, M. genitalium loads were significantly higher in men with NCNGU than those in asymptomatic men (P < 0.05). The quantitative assessment of M. genitalium loads by the TaqMan assay will provide useful information for understanding the pathogenicity of this mycoplasma in the urogenital tract.

* Corresponding author. Mailing address: Department of Urology, Gifu University School of Medicine, 40 Tsukasa-Machi, Gifu City, Gifu 500-8705, Japan. Phone: 81-58-265-1241. Fax: 81-58-265-9009. E-mail: deguchit{at}cc.gifu-u.ac.jp.

Journal of Clinical Microbiology, April 2002, p. 1451-1455, Vol. 40, No. 4
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.4.1451-1455.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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