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Journal of Clinical Microbiology, April 2002, p. 1514-1517, Vol. 40, No. 4
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.4.1514-1517.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Evaluation of a Triplex PCR Assay To Discriminate Staphylococcus aureus from Coagulase-Negative Staphylococci and Determine Methicillin Resistance from Blood Cultures

N. Maes,^* J. Magdalena,^, S. Rottiers, Y. De Gheldre, and M. J. Struelens

Center for Molecular Diagnostics, Service de Microbiologie, Hôpital Erasme, Université Libre de Bruxelles, Brussels, Belgium

Received 23 August 2001/ Returned for modification 14 October 2001/ Accepted 13 January 2002

A triplex PCR targeting the 16S rRNA, mecA, and nuc genes was developed for identification of staphylococci and detection of methicillin resistance. After validation of the assay with a collection of strains of staphylococci and enterococci (n = 169), the assay was evaluated with cultures of blood with gram-positive cocci from 40 patients. Accurate results were obtained for 59 (98%) of 61 cultures within 6 h of growth detection.

Present address: Division of Molecular Cell Biology, School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom.

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