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Journal of Clinical Microbiology, April 2002, p. 1534-1537, Vol. 40, No. 4
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.4.1534-1537.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Detection and Characterization of Rotaviruses in Hospitalized Neonates in Blantyre, Malawi

Wellcome Trust Research Laboratories, College of Medicine, Universities of Malawi and Liverpool,¹ Department of Paediatrics, University of Malawi College of Medicine, Blantyre, Malawi,² Department of Gastroenterology and Clinical Nutrition, Royal Children's Hospital, Melbourne, Australia,⁴ Department of Medical Microbiology and Genito-Urinary Medicine, University of Liverpool, Liverpool, United Kingdom³

Received 21 August 2001/ Returned for modification 18 December 2001/ Accepted 19 January 2002

In five separate fecal collections spanning three years, group A rotaviruses were detected by enzyme-linked immunosorbent assay in 35 (25%) of 142 specimens obtained from nondiarrheic, hospitalized neonates in Blantyre, Malawi. Molecular characterization of each strain identified, for the first time in neonates, a short electropherotype, genotype P[6], G8 strain type, similar to the dominant, cocirculating community strain detected in symptomatic infants in Blantyre. Partial sequence analysis of the VP4 and NSP4 genes of neonatal and community strains failed to identify changes which could explain the differences in clinical outcome. Neonatal serotype G8 rotaviruses should be considered as potential rotavirus vaccine candidates for use in Malawi.

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