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Journal of Clinical Microbiology, May 2002, p. 1592-1602, Vol. 40, No. 5
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.5.1592-1602.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Variable-Number Tandem Repeat Typing of Mycobacterium tuberculosis Isolates with Low Copy Numbers of IS6110 by Using Mycobacterial Interspersed Repetitive Units

Lauren Steinlein Cowan,1 Laura Mosher,2 Lois Diem,1 Jeffrey P. Massey,2 and Jack T. Crawford1*

Division of AIDS, STD, and TB Laboratory Research, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333,1 Molecular Biology Section, Michigan Department of Community Health, Lansing, Michigan 489092

Received 9 November 2001/ Returned for modification 20 January 2002/ Accepted 5 February 2002

A study set of 180 Mycobacterium tuberculosis and Mycobacterium bovis isolates having low copy numbers of IS6110 were genotyped using the recently introduced method based on the variable-number tandem repeats of mycobacterial interspersed repetitive units (MIRU-VNTR). The results were compared with results of the more commonly used methods, IS6110 restriction fragment length polymorphism (RFLP) and spoligotyping. The isolates were collected in Michigan from 1996 to 1999 as part of a project to genotype all isolates from new cases of tuberculosis in the state. Twelve MIRU loci were amplified, and the amplicons were analyzed by agarose gel electrophoresis to determine the copy number at each MIRU locus. MIRU-VNTR produced more distinct patterns (80 patterns) than did IS6110 RFLP (58 patterns), as would be expected in this study set. Spoligotyping identified 59 patterns. No single method defined all unique isolates, and the combination of all three typing methods generated 112 distinct patterns identifying 90 unique isolates and 90 isolates in 22 clusters. The results confirm the potential utility of MIRU-VNTR typing and show that typing with multiple methods is required to attain maximum specificity.

* Corresponding author. Mailing address: Mailstop F08, NCID/DASTLR, CDC, 1600 Clifton Rd., Atlanta, GA 30333. Phone: (404) 639-1281. Fax: (404) 639-1287. E-mail: JCrawford{at}cdc.gov.

Journal of Clinical Microbiology, May 2002, p. 1592-1602, Vol. 40, No. 5
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.5.1592-1602.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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