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Journal of Clinical Microbiology, May 2002, p. 1749-1754, Vol. 40, No. 5
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.5.1749-1754.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

PreservCyt Transport Medium Used for the ThinPrep Pap Test Is a Suitable Medium for Detection of Chlamydia trachomatis by the COBAS Amplicor CT/NG Test: Results of a Preliminary Study and Future Implications

Anne Bianchi,1 François Moret,2 Jean-Marc Desrues,3 Thierry Champenois,3 Yves Dervaux,4 Orlane Desvouas,3 André Oursin,3 Dominique Quinzat,5 Roger Dachez,5 Christian Bathelier,3 and Christophe Ronsin3*

Laboratoire Départemental de Seine-Saint-Denis, Conseil Général, Bondy,1 Roche Diagnostics, Meylan,2 LCL Service de Biologie Moléculaire, Ivry sur Seine,3 Cytyc France SARL, Villepinte,,4 Laboratoire Claude Lévy, Paris, France5

Received 13 August 2001/ Returned for modification 22 November 2001/ Accepted 24 January 2002

The commercial COBAS Amplicor CT/NG test (Roche Diagnostic Systems, Meylan, France) is a sensitive and specific method for detection of Chlamydia trachomatis infections. This test currently consists of using a nucleic acid amplification method to detect C. trachomatis in first-void urine specimens and in endocervical swabs collected in 2-sucrose-phosphate (2SP) transport medium. We conducted a prospective study to determine whether the automated COBAS Amplicor CT/NG test can detect C. trachomatis in cervical specimens collected in PreservCyt transport medium (ThinPrep Pap Test; Cytyc Corporation, Boxborough, Mass.). PreservCyt medium is used to preserve cervical samples before the preparation of ThinPrep slides. We collected 1,000 cervical specimens from young women (age range, 15 to 25 years) during routine Pap smear tests. Only specimens with normal cytology and in which the gynecologist found no clinical evidence of urogenital infections were selected. The samples were stored in PreservCyt transport medium at 15 to 20°C. C. trachomatis was detected in 22 of the 1,000 cervical specimens that had been stored in PreservCyt. To confirm the positive samples, the test was repeated on new endocervical swab specimens collected in 2SP transport medium. Only 9 of the 22 positive patients agreed to undergo this control, but all 9 retested positive. To evaluate the influence of storage conditions on the sensitivity of the C. trachomatis PCR test, all of the positive samples were stored at 15 to 20°C in PreservCyt transport medium and were retested every 2 weeks for 6 weeks. C. trachomatis was successfully amplified from all 22 specimens for the whole 6-week period. The prevalence of C. trachomatis infection was 2.2% in our study population. These results demonstrate that PreservCyt transport medium is a suitable transport medium for detection of C. trachomatis by the COBAS Amplicor CT/NG test. The ThinPrep Pap Test may enable gynecologists to monitor for both cervical lesions and C. trachomatis infections with a single endocervical specimen.

* Corresponding author. Mailing address: L.C.L., Service de Biologie Moléculaire, 78 Avenue de Verdun, BP 110, 94208 Ivry sur Seine, France. Phone: 01-49-59-17-10. Fax: 01-49-59-17-98. E-mail: c.ronsin{at}lablcl.com.

Journal of Clinical Microbiology, May 2002, p. 1749-1754, Vol. 40, No. 5
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.5.1749-1754.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sevestre, H., Mention, J., Lefebvre, J.-F., Eb, F., Hamdad, F. (2009). Assessment of Chlamydia trachomatis infection by Cobas Amplicor PCR and in-house LightCycler assays using PreservCyt and 2-SP media in voluntary legal abortions. J Med Microbiol 58: 59-64 [Abstract] [Full Text]  
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