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Journal of Clinical Microbiology, May 2002, p. 1791-1797, Vol. 40, No. 5
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.5.1791-1797.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Development and Application of a New Scheme for Typing Campylobacter jejuni and Campylobacter coli by PCR-Based Restriction Fragment Length Polymorphism Analysis

Feng Shi,1* Yuen Yuen Chen,1 Trudy M. Wassenaar,2 Walter H. Woods,3 Peter J. Coloe,1 and Benjamin N. Fry1

Department of Biotechnology and Environmental Biology, Royal Melbourne Institute of Technology University, Melbourne, Victoria 3083,1 School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, South Australia, 5001, Australia,3 Molecular Microbiology and Genomics Consultants, Zotzenheim, Germany2

Received 28 November 2001/ Returned for modification 10 January 2002/ Accepted 3 March 2002

A molecular typing approach for Campylobacter jejuni and Campylobacter coli was developed with restriction fragment length polymorphism analysis of a 9.6-kb PCR-amplified portion of the lipopolysaccharide gene cluster. Sixty-one Penner serotype reference strains were analyzed with this new genotyping scheme, and 32 genogroups were found. Eleven additional genogroups were obtained from 87 clinical C. jejuni strains tested. This molecular typing method shows a correlation with the Penner heat-stable serotyping method, a phenotypic typing method based on lipopolysaccharide structures that is often used as a "gold standard" for subtyping Campylobacter spp. This strong correlation suggests that the data obtained can be directly compared with epidemiological data collected in the past by classical serotyping of C. jejuni and C. coli. In contrast to the high percentage of nontypeability by phenotyping, this molecular typing method results in 100% typeability and provides a superior alternative to serotyping.

* Corresponding author. Mailing address: Dept. of Biotechnol. and Environ. Biol., Royal Melbourne Inst. of Technol. Univ., Bundoora W. Campus, Bldg. 223, Lvl. 1, Plenty Rd., Bundoora 3083 VIC, Melbourne, Australia. Phone: 61-3-992-57132. Fax: 61-3-992-57110. E-mail: shi.feng{at}rmit.edu.au.

Journal of Clinical Microbiology, May 2002, p. 1791-1797, Vol. 40, No. 5
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.5.1791-1797.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nakari, U.-M., Laaksonen, K., Korkeila, M., Siitonen, A. (2005). Comparative Typing of Campylobacter jejuni by Heat-Stable Serotyping and PCR-Based Restriction Fragment Length Polymorphism Analysis. J. Clin. Microbiol. 43: 1166-1170 [Abstract] [Full Text]  
  • Lastovica, A. J., Shi, F., Fry, B. N. (2003). Molecular Typing of Campylobacter jejuni and Campylobacter coli. J. Clin. Microbiol. 41: 1349-1350 [Full Text]  


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