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Journal of Clinical Microbiology, May 2002, p. 1821-1823, Vol. 40, No. 5
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.5.1821-1823.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Rapid PCR-Based Identification of Methicillin-Resistant Staphylococcus aureus from Screening Swabs

National Reference Center of Hygiene, Institute of Environmental Medicine and Hospital Epidemiology, University Hospital Freiburg, 79106 Freiburg, Germany,¹ Department of Microbiology, University Hospital, Queens Medical Centre, Nottingham NG7 2UH, United Kingdom²

Received 9 April 2001/ Returned for modification 13 August 2001/ Accepted 13 February 2002

A PCR identification of methicillin-resistant Staphylococcus aureus (MRSA), obviating the need for subculture on agar media, was investigated. The combination of MRSA detection by mecA femB PCR with prior enrichment in selective broth was tested for 439 swabs. PCR identified 36 MRSA-positive samples, in concordance with conventional methods.

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