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Journal of Clinical Microbiology, May 2002, p. 1838-1839, Vol. 40, No. 5
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.5.1838-1839.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Comparison of Chromogenic and Fluorogenic Membrane Filtration Methods for Detection of Four Candida Species

T. G. Bauters and H. J. Nelis^*

Laboratory for Pharmaceutical Microbiology, Department of Pharmaceutical Analysis, Ghent University, Ghent, Belgium

Received 31 August 2001/ Returned for modification 7 October 2001/ Accepted 25 February 2002

CHROMagar Candida medium was inoculated with a variety of clinical samples (n = 282) by using membrane filtration instead of swabbing or streaking. This modified CHROMagar procedure increased the ability to detect yeast by 16.7%. Compared to an enzymatic two-step membrane filtration method with fluorogenic substrates, it yielded similar recovery but was slower.

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* Corresponding author. Mailing address: Laboratory for Pharmaceutical Microbiology, Department of Pharmaceutical Analysis, Harlebekestraat 72, 9000 Ghent, Belgium. Phone: 32-9.2648091. Fax: 32-9.2648195. E-mail: Hans.Nelis{at}rug.ac.be.

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Journal of Clinical Microbiology, May 2002, p. 1838-1839, Vol. 40, No. 5
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.5.1838-1839.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.