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Journal of Clinical Microbiology, May 2002, p. 1869-1872, Vol. 40, No. 5
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.5.1869-1872.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Evidence of the Presence of IS1245 and IS1311 or Closely Related Insertion Elements in Nontuberculous Mycobacteria outside of the Mycobacterium avium Complex

Andreas P. Keller,1 Marjorie L. Beggs,2 Beate Amthor,1 Fabian Bruns,1,{dagger} Peter Meissner,1 and Walter H. Haas1,3*

Department of General Pediatrics, Children's Hospital, University of Heidelberg, 69120 Heidelberg,1 Center for Infectious Disease Epidemiology, Robert Koch-Institute, 10963 Berlin, Germany,3 Department of Pathology, University of Arkansas for Medical Sciences and J. L. McClellan Memorial Veterans Hospital, Little Rock, Arkansas 722052

Received 26 July 2001/ Returned for modification 9 November 2001/ Accepted 25 February 2002

A PCR assay based on the simultaneous detection of IS1245 and IS1311 was developed and used to determine the host range of these insertion elements. Specific PCR products were observed in Mycobacterium malmoense, Mycobacterium scrofulaceum, and Mycobacterium nonchromogenicum, indicating that IS1245 and IS1311 are not limited to the Mycobacterium avium complex.

* Corresponding author. Mailing address: Center for Infectious Disease Epidemiology, Robert Koch-Institut, Stresemannstrasse 90, 10963 Berlin, Germany. Phone: 49 (0) 30 4547-3431. Fax: 49 (0) 30 4547-3533. E-mail: haasw{at}rki.de.

{dagger} Deceased.

Journal of Clinical Microbiology, May 2002, p. 1869-1872, Vol. 40, No. 5
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.5.1869-1872.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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