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Journal of Clinical Microbiology, May 2002, p. 1873-1874, Vol. 40, No. 5
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.5.1873-1874.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Tetrazolium Microplate Assay as a Rapid and Inexpensive Colorimetric Method for Determination of Antibiotic Susceptibility of Mycobacterium tuberculosis

Luz Caviedes,1 Jose Delgado,2 and Robert H. Gilman3*

Infectious Diseases Laboratory, Pathology Department, Universidad Peruana Cayetano Heredia,1 Asociación Benéfica PRISMA, Lima, Peru,2 Department of International Health, Johns Hopkins School of Public Health, Baltimore, Maryland3

Received 24 October 2001/ Returned for modification 4 December 2001/ Accepted 12 February 2002

The emergence of multidrug-resistant tuberculosis underscores the need for low-cost, rapid methods to determine the susceptibility of Mycobacterium tuberculosis to antibiotics. A new, rapid, easily read, and inexpensive colorimetric method with a tetrazolium indicator performs this determination as quickly and accurately as the more expensive Alamar Blue technique.

* Corresponding author. Mailing address: Department of International Health, Johns Hopkins School of Public Health, 615 N. Wolfe St., Room 3501, Baltimore, MD 21205. Phone: (410) 614-3639. Fax: (410) 614-6060. E-mail: rgilman{at}jhsph.edu.

Journal of Clinical Microbiology, May 2002, p. 1873-1874, Vol. 40, No. 5
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.5.1873-1874.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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