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Journal of Clinical Microbiology, June 2002, p. 2041-2045, Vol. 40, No. 6
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.6.2041-2045.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Detection of Antibodies Specific to an Antigenic Cell Wall Galactomannoprotein for Serodiagnosis of Aspergillus fumigatus Aspergillosis

Che-man Chan,1 Patrick C. Y. Woo,1 Andy S. P. Leung,1 Susanna K. P. Lau,1 Xiao-yan Che,1 Liang Cao,1 and Kwok-yung Yuen1,2*

Department of Microbiology, The University of Hong Kong, Queen Mary Hospital,1 HKU-Pasteur Research Centre, Hong Kong2

Received 14 December 2001/ Returned for modification 2 February 2002/ Accepted 6 March 2002

Aspergilloma and invasive aspergillosis are important opportunistic infections caused by Aspergillus species, among which Aspergillus fumigatus is the most common species associated with human disease. We developed an enzyme-linked immunosorbent assay (ELISA)-based antibody assay with Afmp1p, a purified recombinant antigenic cell wall galactomannoprotein of A. fumigatus. Evaluation of the test with guinea pig sera against A. fumigatus and other pathogenic fungi indicated that this assay was specific for A. fumigatus. Clinical evaluation revealed that the assay was 100% sensitive for patients with aspergilloma and 33.3% sensitive for patients with invasive aspergillosis. No false-positive results were found for serum samples from 80 healthy blood donors, 6 patients with typhoid fever, 4 patients with melioidosis, 20 patients with penicilliosis marneffei, 5 patients with candidiasis, and 4 patients with cryptococcosis, indicating a high specificity of the test. Thus, this ELISA-based test for the detection of anti-Afmp1p antibody can be of significant value as a diagnostic for aspergillosis.

* Corresponding author. Mailing address: Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong. Phone: (852) 28554892. Fax: (852) 28551241. E-mail: hkumicro{at}hkucc.hku.hk.

Journal of Clinical Microbiology, June 2002, p. 2041-2045, Vol. 40, No. 6
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.6.2041-2045.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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