This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Montesinos, I.
Right arrow Articles by Sierra, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Montesinos, I.
Right arrow Articles by Sierra, A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, June 2002, p. 2119-2125, Vol. 40, No. 6
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.6.2119-2125.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Epidemiologic Genotyping of Methicillin-Resistant Staphylococcus aureus by Pulsed-Field Gel Electrophoresis at a University Hospital and Comparison with Antibiotyping and Protein A and Coagulase Gene Polymorphisms

I. Montesinos,1* E. Salido,2 T. Delgado,1 M. Cuervo,1 and A. Sierra1

Microbiology Laboratory,1 Research Unit, Hospital Universitario de Canarias, Tenerife, Canary Islands, Spain2

Received 6 November 2001/ Returned for modification 14 January 2002/ Accepted 3 March 2002

A total of 124 methicillin-resistant Staphylococcus aureus (MRSA) isolates were ascertained at the University Hospital of the Canary Islands between January 1997 and April 2000. Genotyping included pulsed-field gel electrophoresis (PFGE) (SmaI digestion) and PCR-restriction fragment length polymorphism (PCR-RFLP) analysis for the coagulase (coa) and protein A (spa) genes. Antibiotic resistance was the main phenotypic marker correlated with genotyping results. Three main PFGE types were detected: A (with 12 subtypes), B (with 2 subtypes), and C. PFGE type A1 was the most commonly found (61% of isolates) and the one responsible for all the epidemic outbreaks. Other genetics markers used (coa and spa RFLPs) were significantly correlated with the PFGE types detected (P < 0.001). These PCR-RFLP assays were useful as molecular markers for a quick, preliminary study of MRSA outbreaks.

* Corresponding author. Mailing address: Servicio de Microbiología, Hospital Universitario, Canarias, Ofra s/n, La Cuesta, La Laguna, S/C de Tenerife, Islas Canarias, Spain. Phone: 34 922 679065. Fax: 34 922 319378. E-mail: carlotamontesin{at}terra.es.

Journal of Clinical Microbiology, June 2002, p. 2119-2125, Vol. 40, No. 6
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.6.2119-2125.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ciftci, A., Onuk, E. E., Findik, A., Yildirim, T., Sogut, M. U. (2009). Molecular typing of Staphylococcus aureus strains from ovine mastitis by pulsed-field gel electrophoresis and polymerase chain reaction based on coagulase and protein A gene polymorphisms. jvdi 21: 849-853 [Abstract] [Full Text]  
  • Strommenger, B., Braulke, C., Heuck, D., Schmidt, C., Pasemann, B., Nubel, U., Witte, W. (2008). spa Typing of Staphylococcus aureus as a Frontline Tool in Epidemiological Typing. J. Clin. Microbiol. 46: 574-581 [Abstract] [Full Text]  
  • Petersdorf, S., Oberdorfer, K., Wendt, C. (2006). Longitudinal Study of the Molecular Epidemiology of Methicillin-Resistant Staphylococcus aureus at a University Hospital. J. Clin. Microbiol. 44: 4297-4302 [Abstract] [Full Text]  
  • Francois, P., Huyghe, A., Charbonnier, Y., Bento, M., Herzig, S., Topolski, I., Fleury, B., Lew, D., Vaudaux, P., Harbarth, S., van Leeuwen, W., van Belkum, A., Blanc, D. S., Pittet, D., Schrenzel, J. (2005). Use of an Automated Multiple-Locus, Variable-Number Tandem Repeat-Based Method for Rapid and High-Throughput Genotyping of Staphylococcus aureus Isolates. J. Clin. Microbiol. 43: 3346-3355 [Abstract] [Full Text]  
  • Shutt, C. K., Pounder, J. I., Page, S. R., Schaecher, B. J., Woods, G. L. (2005). Clinical Evaluation of the DiversiLab Microbial Typing System Using Repetitive-Sequence-Based PCR for Characterization of Staphylococcus aureus Strains. J. Clin. Microbiol. 43: 1187-1192 [Abstract] [Full Text]  
  • Harmsen, D., Claus, H., Witte, W., Rothganger, J., Claus, H., Turnwald, D., Vogel, U. (2003). Typing of Methicillin-Resistant Staphylococcus aureus in a University Hospital Setting by Using Novel Software for spa Repeat Determination and Database Management. J. Clin. Microbiol. 41: 5442-5448 [Abstract] [Full Text]  
  • Stranden, A., Frei, R., Widmer, A. F. (2003). Molecular Typing of Methicillin-Resistant Staphylococcus aureus: Can PCR Replace Pulsed-Field Gel Electrophoresis?. J. Clin. Microbiol. 41: 3181-3186 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»