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Journal of Clinical Microbiology, June 2002, p. 2176-2181, Vol. 40, No. 6
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.6.2176-2181.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Genetic Identification of Rickettsiae Isolated from Ticks in Japan

Pierre-Edouard Fournier,1 Hiromi Fujita,2 Nobuhiro Takada,3 and Didier Raoult1*

Unité des Rickettsies, Faculté de Médecine, CNRS UMR 6020 27, Université de la Méditerranée, 13385 Marseille, Cedex 05, France,1 Ohara Research Laboratory, Ohara General Hospital, Fukushima 960-0195,2 Department of Immunology and Medical Zoology, Fukui Medical University, Matsuoka, Fukui 910-1193, Japan3

Received 7 January 2002/ Returned for modification 2 March 2002/ Accepted 24 March 2002

Following the description in Japan of Japanese spotted fever, caused by Rickettsia japonica, a search for the vector of this disease led to the isolation of several rickettsiae from various tick species. Sixty-three rickettsial isolates were obtained from six different tick species, and six type strains were described by PCR and monoclonal antibody testing. We identified these six strains by amplification and sequencing of the genes encoding 16S rRNA and citrate synthase. We confirmed that the isolates from Dermacentor taiwanensis and Haemaphysalis flava ticks were R. japonica isolates. In Ixodes ovatus, Ixodes persulcatus, and Ixodes monospinosus, we identified a Rickettsia identical or closely related to Rickettsia helvetica, a species that is pathogenic for humans and that to date has only been found in Europe. Finally, we identified a new genotype of unknown pathogenicity, genotype AT, that was isolated from Amblyomma testudinarium ticks and that is closely related to a Slovakian genotype obtained from Ixodes ricinus ticks.

* Corresponding author. Mailing address: Unité des Rickettsies, Faculté de Médecine, CNRS UMR 6020, Université de la Méditerranée, 27, Blvd. Jean Moulin, 13385 Marseille, Cedex 05, France. Phone: 33 4 91 32 43 75. Fax: 33 4 91 38 77 72. E-mail: Didier.Raoult{at}medecine.univ-mrs.fr.

Journal of Clinical Microbiology, June 2002, p. 2176-2181, Vol. 40, No. 6
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.6.2176-2181.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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