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Journal of Clinical Microbiology, June 2002, p. 2199-2206, Vol. 40, No. 6
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.6.2199-2206.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Genetic Structure of Candida glabrata Populations in AIDS and Non-AIDS Patients

Centre d'Étude du Polymorphisme des Microorganismes, UMR 9926 CNRS-IRD, 34000 Montpellier Cedex 1,¹ Laboratoire d'Immunologie et de Parasitologie, Faculté de Pharmacie, 34060 Montpellier Cedex 1,² Clinique des Maladies Infectieuses A, Hôpital Gui de Chauliac, 34000 Montpellier,³ Unité de Parasitologie-Mycologie, Hôpital Louis Mourier, 92700 Colombes, France⁴

Received 5 February 2001/ Returned for modification 4 May 2001/ Accepted 29 January 2002

The genotypes of 63 strains (11 reference strains and 52 strains from hospitalized patients) of the haploid yeast Candida glabrata were determined from 33 putative gene enzymatic loci. This enabled the characterization of 26 different multilocus genotypes. Genetic differentiation was found between distant hospitals (located in Montpellier and Paris, France) but not for other parameters (anatomic origins or human immunodeficiency virus-positive [HIV⁺] and HIV^- patients). Strong nonrandom association between loci could be seen. Such statistical linkages were confirmed upon comparing the patterns of 14 RAPD [random(ly) amplified polymorphic DNA] primers from 20 of these strains to results obtained from multilocus enzyme electrophoresis analysis. This finding suggests a mainly clonal mode of reproduction of C. glabrata. The consequences of the clonality displayed by C. glabrata populations on the epidemiology of this yeast are also discussed.

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