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Journal of Clinical Microbiology, June 2002, p. 2213-2216, Vol. 40, No. 6
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.6.2213-2216.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Rapid Typing of Bordetella pertussis Pertussis Toxin Gene Variants by LightCycler Real-Time PCR and Fluorescence Resonance Energy Transfer Hybridization Probe Melting Curve Analysis

Johanna Mäkinen,^1,2* Jussi Mertsola,³ Matti K. Viljanen,⁴ Heikki Arvilommi,¹ and Qiushui He¹

National Public Health Institute, Department in Turku,¹ Turku Graduate School of Biomedical Sciences,² Departments of Pediatrics,³ Medical Microbiology, University of Turku, Turku, Finland⁴

Received 10 December 2001/ Returned for modification 23 February 2002/ Accepted 29 March 2002

A LightCycler real-time PCR hybridization probe assay was developed for rapid typing of gene variants of the Bordetella pertussis virulence factor pertussis toxin. The assay correctly identified the ptxS1 alleles of all strains tested, comprising 57 Finnish clinical isolates and 2 vaccine strains. The method is simple, reliable, and suitable for large-scale screening of B. pertussis strains.

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* Corresponding author. Mailing address: Pertussis Reference Laboratory, National Public Health Institute, Kiinamyllynkatu 13, 20520 Turku, Finland. Phone: 358-2-251 9255. Fax: 358-2-251 9254. E-mail: johanna.makinen{at}utu.fi.

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Journal of Clinical Microbiology, June 2002, p. 2213-2216, Vol. 40, No. 6
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.6.2213-2216.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

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