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Journal of Clinical Microbiology, June 2002, p. 2297-2299, Vol. 40, No. 6
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.6.2297-2299.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Detection of Mycobacterium avium in Blood Samples of Patients with AIDS by Using PCR

Vívian de F. Sumnienski Rodrigues,1,2 Fernanda C. Queiroz Mello,3 Marta Osório Ribeiro,2 Leila Fonseca,3 Afrânio Lineu Kritski,3 Maria Lucia Rossetti,2 and Arnaldo Zaha1*

Centro de Biotecnologia do Estado do Rio Grande do Sul, Universidade Federal do Rio Grande do Sul,1 Laboratório de Biologia Molecular, Laboratório Central de Saúde Pública do Rio Grande do Sul, Porto Alegre,2 Hospital Universitário Clementino Fraga Filho, Serviço de Pneumologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil3

Received 26 November 2001/ Returned for modification 22 January 2002/ Accepted 4 April 2002

Sixty-nine blood samples from 47 patients infected with human immunodeficiency virus were analyzed by using PCR to detect Mycobacterium avium. The sensitivity can be up to 95.7%, depending on the detection method used and the number of blood samples analyzed from each patient. The procedure can be helpful in the diagnosis of mycobacterial disease.

* Corresponding author. Mailing address: Centro de Biotecnologia/UFRGS, Av. Bento Gonçalves 9500, Prédio 43421, CEP: 91501-970-Porto Alegre, RS, Brazil. Phone: 55 51 3316-6054. Fax: 55 51 3316-7309. E-mail: zaha{at}dna.cbiot.ufrgs.br.

Journal of Clinical Microbiology, June 2002, p. 2297-2299, Vol. 40, No. 6
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.6.2297-2299.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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