This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hamilton, M. S.
Right arrow Articles by Achyuthan, K. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hamilton, M. S.
Right arrow Articles by Achyuthan, K. E.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, July 2002, p. 2331-2334, Vol. 40, No. 7
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.7.2331-2334.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Clinical Evaluation of the ZstatFlu-II Test: a Chemiluminescent Rapid Diagnostic Test for Influenza Virus

Marilyn S. Hamilton,1,2* David M. Abel,2 Yolanda J. Ballam,2 Mary K. Otto,2 Angela F. Nickell,2 Lisa M. Pence,3,{dagger} James R. Appleman,3,{ddagger} Craig D. Shimasaki,3 and Komandoor E. Achyuthan3

University of Missouri—Kansas City School of Medicine,1 The Children's Mercy Hospital, Kansas City, Missouri,2 ZymeTx, Inc., Oklahoma City, Oklahoma3

Received 15 January 2002/ Returned for modification 1 March 2002/ Accepted 5 April 2002

Exploiting the high sensitivity of the chemiluminescence phenomenon, an accurate and sensitive point-of-care test, called the ZstatFlu-II test (ZymeTx, Inc., Oklahoma City, Okla.), was developed to detect influenza virus infections. The ZstatFlu-II test takes 20 min and requires approximately 2 min of "hands-on" time for operational steps. The ZstatFlu-II test does not distinguish between infections with influenza virus types A and B. ZstatFlu-II test results are printed on Polaroid High-Speed Detector Film, allowing test results to be archived. A prototype version of the ZstatFlu-II test was evaluated during the 2000-to-2001 flu season with 300 nasal aspirate specimens from children at a pediatric hospital. Compared to culture, the ZstatFlu-II test had 88% sensitivity and 92% specificity. The Directigen test had a sensitivity of 75% and a specificity of 93%. The sensitivity of the ZstatFlu-II test was significantly higher than that of the Directigen test (P < 0.0574).

* Corresponding author. Mailing address: The Children's Mercy Hospital, Department of Pathology, 2401 Gillham Rd., Kansas City, MO 64108. Phone: (816)-234-3234. Fax: (816) 802-1492. E-mail: mhamilton{at}cmh.edu.

{dagger} Present address: National Center for Toxicological Research, Jefferson, AR 72079.

{ddagger} Present address: Anadys Pharmaceuticals, Inc., San Diego, CA 92121.

Journal of Clinical Microbiology, July 2002, p. 2331-2334, Vol. 40, No. 7
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.7.2331-2334.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Weinberg, A., Walker, M. L. (2005). Evaluation of Three Immunoassay Kits for Rapid Detection of Influenza Virus A and B. CVI 12: 367-370 [Abstract] [Full Text]  
  • Dunn, J. J., Gordon, C., Kelley, C., Carroll, K. C. (2003). Comparison of the Denka-Seiken INFLU A{middle dot}B-Quick and BD Directigen Flu A+B Kits with Direct Fluorescent-Antibody Staining and Shell Vial Culture Methods for Rapid Detection of Influenza Viruses. J. Clin. Microbiol. 41: 2180-2183 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»