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Journal of Clinical Microbiology, July 2002, p. 2346-2351, Vol. 40, No. 7
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.7.2346-2351.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Characterization of Salmonella Serovars by PCR-Single-Strand Conformation Polymorphism Analysis

Satheesh Nair,1,4* Thong Kwai Lin,2 Tikki Pang,3 and Martin Altwegg4

Institute of Postgraduate Studies & Research,1 Institute of Biological Sciences, University of Malaya, Kuala Lumpur, Malaysia,2 World Health Organization, Geneva, Switzerland,3 Department of Medical Microbiology, University of Zurich, Zurich, Switzerland4

Received 25 October 2001/ Returned for modification 16 February 2002/ Accepted 29 March 2002

PCR-restriction fragment length polymorphism (PCR-RFLP) and PCR-single-strand conformation polymorphism (PCR-SSCP) analyses were carried out on the 1.6-kb groEL gene from 41 strains of 10 different Salmonella serovars. Three HaeIII RFLP profiles were recognized, but no discrimination between the serovars could be achieved by this technique. However, PCR-SSCP analysis of the groEL genes of various Salmonella serovars produced 14 SSCP profiles, indicating the potential of this technique to differentiate different Salmonella serovars (interserovar differentiation). Moreover, PCR-SSCP could differentiate strains within a subset of serovars (intraserovar discrimination), as three SSCP profiles were produced for the 11 Salmonella enterica serovar Enteritidis strains, and two SSCP profiles were generated for the 7 S. enterica serovar Infantis and five S. enterica serovar Newport strains. PCR-SSCP has the potential to complement classical typing methods such as serotyping and phage typing for the typing of Salmonella serovars due to its rapidity, simplicity, and typeability.

* Corresponding author. Present address: Department of Biological Sciences, University of Calgary, 2500 University Dr. N.W., Calgary, Alberta, Canada T2N 1N4. Phone: (403) 220-3572. Fax: (403) 289-9311. E-mail: h0sathe{at}yahoo.com.

Journal of Clinical Microbiology, July 2002, p. 2346-2351, Vol. 40, No. 7
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.7.2346-2351.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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