This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Van Horn, K.
Right arrow Articles by Kumon, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Van Horn, K.
Right arrow Articles by Kumon, H.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, July 2002, p. 2476-2479, Vol. 40, No. 7
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.7.2476-2479.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Evaluation of 15 Motility Media and a Direct Microscopic Method for Detection of Motility in Enterococci

Kenneth Van Horn,1* Clara Tóth,2 Reiko Kariyama,3 Ritsuko Mitsuhata,3 and Hiromi Kumon3

Microbiology, Westchester Medical Center, and New York Medical College, Valhalla, New York 10595,1 Division of Natural Sciences, Mathematics and Computer Sciences, St. Thomas Aquinas College, Sparkill, New York 10976,2 Department of Urology, Okayama University Graduate School of Medicine and Dentistry, Okayama 700-8558, Japan3

Received 26 December 2001/ Returned for modification 4 March 2002/ Accepted 29 April 2002

Isolation of motile, vanC enterococci has yet to be a major infection control concern; however, rapid detection still is important. We evaluated 15 motility media from three manufacturers and a 2-h direct microscopic method for accurate detection of 89 enterococcal strains, including 72 vanC enterococcal strains. Resistance genes were confirmed by a multiplex PCR method with the vanC gene detected in all motile enterococci. Motility in the 72 vanC enterococci was detected at 30°C within 72 h in BD Biosciences motility nitrate medium and in Remel motility B medium, motility B medium supplemented with methyl-{alpha}-D-glucopyranoside (investigational), motility S medium, motility test medium, and motility test medium with tetrazolium indicator. Motility was also observed for all vanC enterococci with the 2-h direct (30°C incubation) microscopic detection method. All Enterococcus faecalis and Enterococcus faecium isolates were observed to be nonmotile in all media and by the direct microscopic method. Since differences between the various motility media tested were observed, the medium used for detection of enterococcal motility must be selected carefully.

* Corresponding author. Mailing address: Microbiology, Westchester Medical Center, Grasslands Rd., Valhalla, NY 10595. Phone and fax: (914) 493-7502. E-mail: vanhornk{at}wcmc.com.

Journal of Clinical Microbiology, July 2002, p. 2476-2479, Vol. 40, No. 7
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.7.2476-2479.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»