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Journal of Clinical Microbiology, July 2002, p. 2480-2482, Vol. 40, No. 7
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.7.2480-2482.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Performance of CHROMagar Selective Medium and Oxacillin Resistance Screening Agar Base for Identifying Staphylococcus aureus and Detecting Methicillin Resistance

Jan Kluytmans,1* Arjanne Van Griethuysen,2 Piet Willemse,1 and Peter Van Keulen1

Department of Microbiology and Infection Control, Amphia Hospital, Breda,1 Department of Clinical Microbiology, Hospital Rijnstate, Arnhem, The Netherlands2

Received 19 February 2002/ Returned for modification 25 March 2002/ Accepted 18 April 2002

Two new selective media, oxacillin resistance screening agar base (ORSAB) and CHROMagar Staph aureus (CSA), were evaluated for identification of Staphylococcus aureus and for screening of methicillin resistance by addition of antimicrobial agents to these media. A well-defined collection consisting of 1,140 staphylococci was used. A total of 624 were S. aureus, of which 358 were methicillin susceptible and 266 were methicillin resistant, and 516 were coagulase-negative staphylococci. The methicillin-resistant S. aureus (MRSA) strains were selected based on the results of phage typing; 247 different types were included in the analysis. For identification of S. aureus, both media performed better after 24 h than after 48 h. The sensitivities at 24 h were comparable (CSA, 98.6%; ORSAB, 97.1%), but the specificity of CSA was significantly higher (CSA, 97.1%; ORSAB, 92.1%). For screening of methicillin resistance, antibiotic supplements were added to both media. The sensitivity was lower after 24 h (CSA, 58.6%; ORSAB, 84.2%) and increased significantly after 48 h (CSA, 77.5%; ORSAB, 91.4%). At both time intervals ORSAB was significantly more sensitive than CSA. However, the specificities of both media were high after 24 h (CSA, 99.1%; ORSAB, 98.3%) and decreased significantly after 48 h of incubation (CSA, 94.7%; ORSAB, 95.5%). In conclusion, for identification of S. aureus, CSA is more accurate than ORSAB because of a significantly higher specificity. For screening of MRSA, ORSAB performs better than CSA, but the usefulness in clinical practice is limited because a significant number of strains are not detected.

* Corresponding author. Mailing address: Department of Microbiology and Infection Control, Amphia Hospital Breda, Location Langendijk, Langendijk 75, 4819 EV Breda, The Netherlands. Phone: 31 76 5277060. Fax: 31 76 5277053. E-mail: jkluytmans{at}amphia.nl.

Journal of Clinical Microbiology, July 2002, p. 2480-2482, Vol. 40, No. 7
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.7.2480-2482.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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