This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ikoma, M.
Right arrow Articles by Welling-Wester, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ikoma, M.
Right arrow Articles by Welling-Wester, S.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, July 2002, p. 2526-2532, Vol. 40, No. 7
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.7.2526-2532.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Use of a Fragment of Glycoprotein G-2 Produced in the Baculovirus Expression System for Detecting Herpes Simplex Virus Type 2-Specific Antibodies

Minako Ikoma,1 Jan-Åke Liljeqvist,2 Jan Groen,3 Koen L. Glazenburg,1 T. Hauw The,4 and Sytske Welling-Wester1*

Department of Medical Microbiology,1 Department of Clinical Immunology, University of Groningen, 9713 GZ Groningen,4 Institute of Virology, Erasmus Medical Center, 3015 GD Rotterdam, The Netherlands,3 Department of Clinical Virology, University of Göteborg, Göteborg, Sweden2

Received 17 October 2001/ Returned for modification 24 January 2002/ Accepted 30 March 2002

Fragments of glycoprotein G (gG-2281-594His), comprising residues 281 to 594 of herpes simplex virus type 2 (HSV-2), glycoprotein G of HSV-1 (gG-1t26-189His), and glycoprotein D of HSV-1 (gD-11-313), were expressed in the baculovirus expression system to develop an assay for the detection of HSV-1 and HSV-2 type-specific antibodies. The expression of the gG-1t26-189His and gG-2281-594His fragments was analyzed by Western blotting using monoclonal antibodies LP10 and AP1, respectively. The molecular masses of the major products of gG-1t26-189His and the fragment of gG-2281-594His were 36 to 39 kDa and 64 to 72 kDa, respectively. Human sera positive for HSV-1 reacted with gG-1t26-189His, sera positive for HSV-2 reacted with the gG-2281-594His fragment, and sera positive for both types reacted with gG-1t26-189His and gG-2281-594His in Western blotting. The human sera recognized polypeptides of gG-2281-594His with molecular masses of 57 to 67 and 120 to 150 kDa and additional faint bands of 21, 29, and 45 kDa. The recombinant gG-1t26-189His and the recombinant gG-2281-594His fragment were used as type-specific antigens for the detection of HSV-1- and HSV-2-specific antibody responses in human sera, respectively. As type-common antigens, an extract of HSV-1-infected Vero cells and recombinant gD-11-313 were used. An enzyme-linked immunosorbent assay to detect type-specific antibodies was developed, and the sensitivity and specificity were evaluated by comparison with commercial tests by using sera obtained from different sources. The sensitivity and specificity were 91.5 and 95.5%, respectively, compared to the Gull assay. The gG-2281-594His fragment can be obtained in relatively large quantities at low cost.

* Corresponding author. Mailing address: Department of Medical Microbiology, University of Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands. Phone: 31-50-3633514. Fax: 31-50-3633528. E-mail: s.welling-wester{at}med.rug.nl.

Journal of Clinical Microbiology, July 2002, p. 2526-2532, Vol. 40, No. 7
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.7.2526-2532.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Perelygina, L., Patrusheva, I., Hombaiah, S., Zurkuhlen, H., Wildes, M. J., Patrushev, N., Hilliard, J. (2005). Production of Herpes B Virus Recombinant Glycoproteins and Evaluation of Their Diagnostic Potential. J. Clin. Microbiol. 43: 620-628 [Abstract] [Full Text]  
  • Wales, S. Q., Smith, C. C., Wachsman, M., Calton, G., Aurelian, L. (2004). Performance and Use of a Ribonucleotide Reductase Herpes Simplex Virus Type-Specific Serological Assay. CVI 11: 42-49 [Abstract] [Full Text]  
  • Gorander, S., Svennerholm, B., Liljeqvist, J.-A. (2003). Secreted Portion of Glycoprotein G of Herpes Simplex Virus Type 2 Is a Novel Antigen for Type-Discriminating Serology. J. Clin. Microbiol. 41: 3681-3686 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»