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Journal of Clinical Microbiology, July 2002, p. 2609-2611, Vol. 40, No. 7
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.7.2609-2611.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Quantitative Stability of DNA after Extended Storage of Clinical Specimens as Determined by Real-Time PCR

Keith R. Jerome,1,2* Meei-Li Huang,1,2 Anna Wald,3,4 Stacy Selke,5 and Lawrence Corey1,2,3

Departments of Laboratory Medicine,1 Medicine,3 and Epidemiology,4 University of Washington, Seattle, Washington 98195, Program in Infectious Diseases, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, and Virology Section, Children’s Hospital and Regional Medical Center, Seattle, Washington 98105,2 and Virology Section, Children’s Hospital and Regional Medical Center, Seattle, Washington 981055

Received 7 December 2001/ Returned for modification 20 January 2002/ Accepted 13 April 2002

Viral DNA stored for extended periods can be amplified by PCR. However, it is unknown whether stored specimens give accurate quantitative results by newer real-time PCR techniques. We therefore compared herpes simplex virus DNA levels in specimens before and after 16 months of storage. The levels of viral DNA remained stable whether the DNA was stored as purified DNA or unextracted DNA in a whole specimen.

* Corresponding author. Mailing address: Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. North, D3-100, Seattle, WA 98109. Phone: (206) 667-6793. Fax: (206) 667-4411. E-mail: kjerome{at}u.washington.edu.

Journal of Clinical Microbiology, July 2002, p. 2609-2611, Vol. 40, No. 7
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.7.2609-2611.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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Copyright © 2002 by the American Society for Microbiology. All rights reserved.