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Journal of Clinical Microbiology, July 2002, p. 2659-2661, Vol. 40, No. 7
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.7.2659-2661.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Rapid Identification of Staphylococcus aureus and the mecA Gene from BacT/ALERT Blood Culture Bottles by Using the LightCycler System

Nabin K. Shrestha,1,2 Marion J. Tuohy,1 Gerri S. Hall,1 Carlos M. Isada,2 and Gary W. Procop1*

Section of Clinical Microbiology,1 Division of Infectious Diseases, The Cleveland Clinic Foundation, Cleveland, Ohio2

Received 28 December 2001/ Returned for modification 10 February 2002/ Accepted 6 April 2002

One hundred BacT/ALERT blood culture bottles growing gram-positive cocci in clusters were cultured and studied by LightCycler PCR for the sa442 and mecA genes. PCR was 100% sensitive and specific for detecting Staphylococcus aureus and methicillin resistance in S. aureus but was less accurate for methicillin resistance in coagulase-negative staphylococci.

* Corresponding author. Mailing address: Clinical Microbiology, Cleveland Clinic Foundation/L40, 9500 Euclid Ave., Cleveland, OH 44195. Phone: (216) 444-5879. Fax: (216) 445-6984. E-mail: procopg{at}ccf.org.

Journal of Clinical Microbiology, July 2002, p. 2659-2661, Vol. 40, No. 7
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.7.2659-2661.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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