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Journal of Clinical Microbiology, July 2002, p. 2671-2674, Vol. 40, No. 7
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.7.2671-2674.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

PCR for Detection of cdt-III and the Relative Frequencies of Cytolethal Distending Toxin Variant-Producing Escherichia coli Isolates from Humans and Cattle

Clifford G. Clark,* Shelley T. Johnson, Russell H. Easy, Jennifer L. Campbell, and Frank G. Rodgers,{dagger}

National Laboratory for Enteric Pathogens, National Microbiology Laboratory, Canadian Science Centre for Human and Animal Health, Winnipeg, Manitoba, Canada

Received 26 November 2001/ Returned for modification 9 February 2002/ Accepted 15 April 2002

A PCR assay that uses primers whose sequences were obtained from the published sequence of the cdt-III gene was developed to determine the frequencies of the cdt-I, cdt-II, and cdt-III genes in Escherichia coli isolates from humans and animals. E. coli isolates producing cytolethal distending toxin (CDT) were infrequently detected. The cdt-I gene was preferentially detected in strains with the cnf1 gene, while the cdt-III gene was found in strains carrying the cnf2 gene. The cdt-III genotype was more prevalent in animal isolates, while the cdt-I and cdt-II genotypes were more evident in human isolates. The presence of further cdt gene variants was indicated by the presence of toxin activity in cell culture in the absence of PCR amplification of the cdt-I, cdt-II, or cdt-III gene.

* Corresponding author. Mailing address: Canadian Science Centre for Human and Animal Health, 1015 Arlington St., Winnipeg, Manitoba, Canada R3E 3R2. Phone: (204) 789-2094. Fax: (204) 789-5012. E-mail: Clifford_Clark{at}hc-sc.gc.ca.

{dagger} Present address: Department of Microbiology, Rudman Hall, University of New Hampshire, Durham, NH 03824.

Journal of Clinical Microbiology, July 2002, p. 2671-2674, Vol. 40, No. 7
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.7.2671-2674.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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