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Journal of Clinical Microbiology, August 2002, p. 2746-2749, Vol. 40, No. 8
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.8.2746-2749.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Vaginal Lactobacillus Flora of Healthy Swedish Women

Laboratory of Food Hygiene, Division of Food Technology, Lund University, SE-221 00 Lund,¹ Division of Clinical Microbiology,² Division of Obstetrics and Gynaecology, Faculty of Health Sciences, Department of Health and Environment, SE-581 85 Linköping, Sweden³

Received 26 December 2001/ Returned for modification 2 March 2002/ Accepted 6 May 2002

Species of the Lactobacillus acidophilus complex are generally considered to constitute most of the vaginal Lactobacillus flora, but the flora varies between studies. However, this may be due to difficulties in identifying the closely related species within the L. acidophilus complex by using traditional methods and to variations in the vaginal status of the participants. Two hundred two isolates from the vaginal fluids of 23 Swedish women without bacterial vaginosis, as defined by the criteria of Nugent et al. (R. P. Nugent, M. A. Krohn, and S. L. Hillier, J. Clin. Microbiol. 29:297-301, 1991), were typed by randomly amplified polymorphic DNA (RAPD) analysis and identified to the species level by temporal temperature gradient gel electrophoresis, multiplex PCR, and 16S ribosomal DNA sequencing. The vaginal flora of most participants was dominated by a single RAPD type, but five of them harbored two RAPD types representing two different species or strains. The most frequently occurring species were Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus iners, and Lactobacillus jensenii. L. iners has not previously been reported as one of the predominant Lactobacillus species in the vagina.

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