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Journal of Clinical Microbiology, August 2002, p. 2854-2859, Vol. 40, No. 8
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.8.2854-2859.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Molecular Epidemiology of Caliciviruses Causing Outbreaks and Sporadic Cases of Acute Gastroenteritis in Spain

J. Buesa,1* B. Collado,1 P. López-Andújar,1 R. Abu-Mallouh,1 J. Rodríguez Díaz,1 A. García Díaz,1 J. Prat,2 S. Guix,3 T. Llovet,4 G. Prats,4 and A. Bosch3

Department of Microbiology, School of Medicine, Hospital Clínico Universitario, University of Valencia, 46010 Valencia,1 Laboratory of Microbiology, Hospital of Sagunto, 46500 Valencia,2 Department of Microbiology, School of Biology, University of Barcelona, 08028 Barcelona,3 Department of Microbiology, Hospital Santa Creu i Sant Pau, Universitat Autónoma de Barcelona, 08025 Barcelona, Spain4

Received 25 March 2002/ Returned for modification 3 May 2002/ Accepted 24 May 2002

The molecular epidemiology of human caliciviruses (HuCVs) causing sporadic cases and outbreaks of acute gastroenteritis around eastern Spain (Catalonia and the Valencian Community) was studied by reverse transcription-PCR (RT-PCR) and by sequencing part of the RNA polymerase gene in open reading frame 1. HuCVs were detected in 44 of 310 stool specimens (14.19%) negative for other enteric pathogens obtained from children with acute gastroenteritis. Norwalk-like viruses (NLVs) were the most common cause of the gastroenteritis outbreaks investigated here. They were detected in 14 out of 25 (56%) outbreaks with an identified pathogen. Genotypes producing both sporadic cases and outbreaks were diverse, with a predominance of GGII strains related to genotypes Melksham and Lordsdale. Five strains clustered with a "new variant" designated GGIIb, which was detected circulating throughout quite a few European countries in the years 2000 and 2001. The emergence mechanism of these strains might be the occurrence of intertypic recombinations between different viruses. The nucleotide sequence of part of the capsid gene (ORF2) from three of these strains demonstrated their relationship with Mexico virus.

* Corresponding author. Mailing address: Departament de Microbiologia, Facultat de Medicina, Universitat de València, Avda. Blasco Ibáñez, 17, 46010 Valencia, Spain. Phone: 34 96 386 46 58. Fax: 34 96 386 41 73. E-mail: javier.buesa{at}uv.es.

Journal of Clinical Microbiology, August 2002, p. 2854-2859, Vol. 40, No. 8
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.8.2854-2859.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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