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Journal of Clinical Microbiology, August 2002, p. 2893-2896, Vol. 40, No. 8
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.8.2893-2896.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

External Quality Assessment Program for Chlamydia trachomatis Diagnostic Testing by Nucleic Acid Amplification Assays

Sally Land,1* Sepehr Tabrizi,2 Anthony Gust,1 Elizabeth Johnson,1 Susan Garland,2 and Elizabeth M. Dax1

National Serology Reference Laboratory, Australia, St Vincent's Institute of Medical Research, Fitzroy, VIC 3065,1 Department of Microbiology and Infectious Diseases, Women's and Children's Health Care Network, Royal Women's Hospital, Carlton, VIC 3053, Australia2

Received 14 December 2001/ Returned for modification 28 January 2002/ Accepted 12 March 2002

We report the results from 57 Australian diagnostic laboratories testing two external quality assessment panels using either the Roche Amplicor Chlamydia trachomatis test (R-PCR) or the Abbott LCx Chlamydia trachomatis assay (A-ligase chain reaction [LCR]). Panel samples were either normal urine spiked with Chlamydia trachomatis antigen or clinical urine specimens. There was no significant difference between laboratories or between assays in detection of C. trachomatis-positive clinical samples. Only at the lower limit of detection of the assays did the R-PCR demonstrate increased sensitivity over the A-LCR in the detection of C. trachomatis antigen. However, it was found that single-sample testing could lead to decreased test sensitivity. Detection of the presence of inhibitors of nucleic acid amplification differed between laboratories.

* Corresponding author. Mailing address: National Serology Reference Laboratory, Australia, at St Vincent's Institute of Medical Research, 41 Victoria Parade, Fitzroy, Victoria, Australia. Phone: 613 9418 1111. Fax: 613 9418 1155. E-mail: sally{at}nrl.gov.au.

Journal of Clinical Microbiology, August 2002, p. 2893-2896, Vol. 40, No. 8
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.8.2893-2896.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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