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Identification of Streptococcus bovis Biotype I Strains among S. bovis Clinical Isolates by PCR

Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305,¹ Protein Design Labs, Inc., Fremont, California 94555,² Clinical Microbiology Laboratories, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114,³ Centers for Disease Control, Atlanta, Georgia 30333⁴

Received 31 January 2002/ Returned for modification 10 April 2002/ Accepted 24 April 2002

Streptococcus bovis causes 24% of all streptococcal infective endocarditis cases. There are many reports linking both S. bovis bacteremia and endocarditis with various forms of gastrointestinal disease (primarily colonic cancers). S. bovis is divided into two biotypes: I and II. The biotype I strain is much more frequently isolated from patients with endocarditis, gastrointestinal disease, or both. We describe here the isolation of biotype I-specific DNA sequences and the development of a PCR test which can identify S. bovis biotype I strains among S. bovis clinical isolates.

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