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Journal of Clinical Microbiology, August 2002, p. 3012-3016, Vol. 40, No. 8
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.8.3012-3016.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Large-Scale Screening of Nasal Swabs for Bacillus anthracis: Descriptive Summary and Discussion of the National Institutes of Health's Experience

Pattarachai Kiratisin,^1* Caroline D. Fukuda,¹ Alexandra Wong,¹ Frida Stock,¹ Jeanne C. Preuss,¹ Laura Ediger,¹ Trupti N. Brahmbhatt,² Steven H. Fischer,¹ Daniel P. Fedorko,¹ Frank G. Witebsky,¹ and Vee J. Gill¹

Microbiology Service, Department of Laboratory Medicine, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892,¹ Clinical Pathology Service, National Naval Medical Center, Bethesda, Maryland 20889²

Received 27 February 2002/ Returned for modification 20 April 2002/ Accepted 18 May 2002

In October 2001, a letter containing a large number of anthrax spores was sent through the Brentwood post office in Washington, D.C., to a United States Senate office on Capitol Hill, resulting in contamination in both places. Several thousand people who worked at these sites were screened for spore exposure by collecting nasal swab samples. We describe here a screening protocol which we, as a level A laboratory, used on very short notice to process a large number of specimens (3,936 swabs) in order to report preliminary results as quickly as possible. Six isolates from our screening met preliminary criteria for Bacillus anthracis identification and were referred for definitive testing. Although none of the isolates was later confirmed to be B. anthracis, we studied these isolates further to define their biochemical characteristics and 16S rRNA sequences. Four of the six isolates were identified as Bacillus megaterium, one was identified as Bacillus cereus, and one was an unidentifiable Bacillus sp. Our results suggest that large-scale nasal-swab screening for potential exposure to anthrax spores, particularly if not done immediately postexposure, may not be very effective for detecting B. anthracis but may detect a number of Bacillus spp. that are phenotypically very similar to B. anthracis.

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* Corresponding author. Mailing address: Microbiology Service, Department of Laboratory Medicine, National Institutes of Health, Building 10, Room 2C385, 10 Center Dr. MSC 1508, Bethesda, MD 20892-1508. Phone: (301) 496-4433. Fax: (301) 402-1886. E-mail: pkiratisin{at}mail.cc.nih.gov.

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Journal of Clinical Microbiology, August 2002, p. 3012-3016, Vol. 40, No. 8
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.8.3012-3016.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

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