JCM Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Selvakumar, N.
Right arrow Articles by Narayanan, P. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Selvakumar, N.
Right arrow Articles by Narayanan, P. R.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, August 2002, p. 3017-3020, Vol. 40, No. 8
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.8.3017-3020.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Evaluation of the Phenol Ammonium Sulfate Sedimentation Smear Microscopy Method for Diagnosis of Pulmonary Tuberculosis

N. Selvakumar,1* Fathima Rahman,1 Renu Garg,2 S. Rajasekaran,3 Nalini Sunder Mohan,1 K. Thyagarajan,1 V. Sundaram,1 T. Santha,1 Thomas R. Frieden,2 and P. R. Narayanan1

Tuberculosis Research Centre (ICMR),1 Government Hospital for Thoracic Medicine, Tambaram Sanatorium, Chennai,3 South East Asia Regional Office, Stop Tuberculosis Unit, World Health Organization, New Delhi, India2

Received 3 March 2002/ Returned for modification 11 April 2002/ Accepted 5 May 2002

We compared the sensitivity and specificity of the phenol ammonium sulfate (PhAS) sediment smear microscopy method for detection of acid-fast bacilli with those of direct smear microscopy, using culture results for Mycobacterium tuberculosis as the "gold standard." The sensitivities of the PhAS and direct smear methods were 85% (465 of 547) and 83% (454 of 547), respectively, and the specificity of each method was 97%. The PhAS method was better accepted by the laboratory technicians and safer but necessitates an overnight sedimentation, which delays reporting of results until 1 day after sputum collection.

* Corresponding author. Mailing address: N. Selvakumar, Tuberculosis Research Centre (ICMR), Mayor V. R. Ramanathan Rd., Chetput, Chennai-600 031, India. Phone: 91 44 8265425. Fax: 91 44 8262137. E-mail: modeldots{at}vsnl.com or selvakumarn{at}hotmail.com.

Journal of Clinical Microbiology, August 2002, p. 3017-3020, Vol. 40, No. 8
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.8.3017-3020.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2002 by the American Society for Microbiology. All rights reserved.