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Journal of Clinical Microbiology, August 2002, p. 3025-3031, Vol. 40, No. 8
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.8.3025-3031.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

DNA Fingerprinting of Pasteurella multocida Recovered from Avian Sources

Alongkorn Amonsin, James F. X. Wellehan,,{dagger} Ling-Ling Li, Judy Laber,,{ddagger} and Vivek Kapur*

Biomedical Genomics Center and Department of Veterinary Pathobiology, University of Minnesota, St. Paul, Minnesota

Received 12 October 2001/ Returned for modification 9 February 2002/ Accepted 12 May 2002

Repetitive sequence-based PCR (rep-PCR) and amplified fragment length polymorphism (AFLP) were used to characterize a sample of 43 field isolates and 4 attenuated vaccine strains of Pasteurella multocida recovered from multiple avian species. Both rep-PCR and AFLP assays were rapid and reproducible, with high indices of discrimination. Concordance analyses of rep-PCR and AFLP with somatic serotyping indicate that, in general, somatic serotyping is a poor indicator of genetic relatedness among isolates of P. multocida. In addition, the data provide evidence of host specificity of P. multocida clones. Overall, the results of our study indicate that the rep-PCR and AFLP techniques enable rapid fingerprinting of P. multocida isolates from multiple avian species and enhance the investigation of fowl cholera outbreaks.

* Corresponding author. Mailing address: University of Minnesota, 1971 Commonwealth Ave., St. Paul, MN 55108. Phone: (612) 625-7712. Fax: (612) 625-5203. E-mail: vkapur{at}umn.edu.

{dagger} Present address: Avian Exotics Service, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, Canada.

{ddagger} Deceased.

Journal of Clinical Microbiology, August 2002, p. 3025-3031, Vol. 40, No. 8
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.8.3025-3031.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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