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Journal of Clinical Microbiology, August 2002, p. 3050-3052, Vol. 40, No. 8
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.8.3050-3052.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Real-Time PCR Detection of Salmonella in Suspect Foods from a Gastroenteritis Outbreak in Kerr County, Texas

Luke T. Daum,1* William J. Barnes,1 James C. McAvin,1 Margaret S. Neidert,2 Lynn A. Cooper,1 William B. Huff,1 Linda Gaul,3 W. S. Riggins,3 Sandra Morris,3 Ann Salmen,1 and Kenton L. Lohman1

Brooks Air Force Base, San Antonio,1 U.S. Army, Texas Department of Health Fellowship,2 Texas Department of Health, Austin, Texas3

Received 1 March 2002/ Returned for modification 21 April 2002/ Accepted 25 May 2002

In June 2001, an outbreak of acute gastroenteritis among 109 attendees of a church picnic in Kerr County, Texas, was reported. A 5'-nuclease PCR assay was used to screen for Salmonella in nine food items from the buffet line. Barbeque chicken B tested positive for Salmonella, and no amplification was detected in the remaining food items. These PCR findings were consistent with culture results and were confirmed by direct nucleotide sequencing. Salmonella enterica serotype Panama was cultured from both food and patient stool samples.

* Corresponding author. Mailing address: Molecular Epidemiology Branch, 2601 Louis Bauer Dr., Brooks AFB, San Antonio, TX 78235-5237. Phone: (210) 536-1687. Fax: (210) 536-2638. E-mail: Luke.Daum{at}brooks.af.mil.

Journal of Clinical Microbiology, August 2002, p. 3050-3052, Vol. 40, No. 8
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.8.3050-3052.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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