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Journal of Clinical Microbiology, August 2002, p. 3066-3067, Vol. 40, No. 8
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.8.3066-3067.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Detection of Enteroaggregative Escherichia coli with Formalin-Preserved HEp-2 Cells

Mohamad S. Miqdady,1 Zhi-Dong Jiang,2 James P. Nataro,3 and Herbert L. DuPont1,2,4*

Baylor College of Medicine,1 Center for Infectious Diseases, University of Texas-Houston, School of Public Health and Medical School,2 St. Luke's Episcopal Hospital, Houston, Texas,4 University of Maryland School of Medicine, Baltimore, Maryland3

Received 25 March 2001/ Returned for modification 23 April 2002/ Accepted 15 May 2002

Formalin-stored HEp-2 cells were used to assay Escherichia coli for adherence. Cells refrigerated in formalin for up to 28 days and used in a wet assay format demonstrated an assay sensitivity ranging from 94 to 98% to detect enteroaggregative E. coli (EAEC). HEp-2 cells first fixed and stored with formalin and then stored dry in ambient conditions for 6 weeks demonstrated an assay sensitivity of 92% to detect EAEC. Using formalin-fixed HEp-2 cells will improve the efficiency of EAEC identification.

* Corresponding author. Mailing address: St. Luke's Episcopal Hospital, 6720 Bertner Ave., MC 1-164, Houston, TX 77030. Phone: (832) 355-4122. Fax: (832) 355-4176. E-mail: hdupont{at}sleh.com.

Journal of Clinical Microbiology, August 2002, p. 3066-3067, Vol. 40, No. 8
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.8.3066-3067.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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