Journal of Clinical Microbiology, August 2002, p. 3089-3092, Vol. 40, No. 8
0095-1137/02/$04.00+0 DOI: 10.1128/JCM.40.8.3089-3092.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.
Performance of an IS6110-Based PCR Assay and the COBAS AMPLICOR MTB PCR System for Detection of Mycobacterium tuberculosis Complex DNA in Human Lymph Node Samples
Dagmar Rimek,1* Sachin Tyagi,2 and Reinhard Kappe3
Department of Medical Microbiology and Hospital Hygiene, University Hospital, D-18057 Rostock,1
Institute of Medical Microbiology and Hygiene, D-99089 Erfurt, Germany,3
Department of Microbiology, Subharati Medical College, Meerut, India2
Received 10 December 2001/
Returned for modification 19 March 2002/
Accepted 18 May 2002
We compared the performance of two PCR assays, an IS6110-based in-house protocol and the COBAS AMPLICOR MTB PCR (COBAS MTB) system, for the detection of Mycobacterium tuberculosis complex in 43 human lymph node samples from 40 patients. For the in-house PCR and the COBAS MTB assays, respectively, sensitivities were 87.5% versus 45.5% (P < 0.05), specificities were 100.0% versus 91.3% (P > 0.05), and inhibition rates were 4.8% versus 19.5% (P < 0.05). For the COBAS MTB system, additional N-acetyl-L-cysteine-NaOH pretreatment of the samples changed neither the inhibition rate nor the sensitivity significantly.
* Corresponding author. Mailing address: Department of Medical Microbiology and Hospital Hygiene, University Hospital, Schillingallee 70, D-18057 Rostock, Germany. Phone: 49-381-4945943. Fax: 49-381-4945902. E-mail: dagmar.rimek{at}med.uni-rostock.de.
Journal of Clinical Microbiology, August 2002, p. 3089-3092, Vol. 40, No. 8
0095-1137/02/$04.00+0 DOI: 10.1128/JCM.40.8.3089-3092.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.
This article has been cited by other articles:
-
Wang, J.-Y., Lee, L.-N., Hsu, H.-L., Hsueh, P.-R., Luh, K.-T.
(2006). Performance Assessment of the DR. MTBC Screen Assay and the BD ProbeTec ET System for Direct Detection of Mycobacterium tuberculosis in Respiratory Specimens.. J. Clin. Microbiol.
44: 716-719
[Abstract]
[Full Text]
-
Wang, J.-Y., Lee, L.-N., Chou, C.-S., Huang, C.-Y., Wang, S.-K., Lai, H.-C., Hsueh, P.-R., Luh, K.-T.
(2004). Performance Assessment of a Nested-PCR Assay (the RAPID BAP-MTB) and the BD ProbeTec ET System for Detection of Mycobacterium tuberculosis in Clinical Specimens. J. Clin. Microbiol.
42: 4599-4603
[Abstract]
[Full Text]
-
Piersimoni, C., Scarparo, C.
(2003). Relevance of Commercial Amplification Methods for Direct Detection of Mycobacterium tuberculosis Complex in Clinical Samples. J. Clin. Microbiol.
41: 5355-5365
[Full Text]
Copyright © 2002 by the American Society for Microbiology. All rights reserved.