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Journal of Clinical Microbiology, August 2002, p. 3089-3092, Vol. 40, No. 8
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.8.3089-3092.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Performance of an IS6110-Based PCR Assay and the COBAS AMPLICOR MTB PCR System for Detection of Mycobacterium tuberculosis Complex DNA in Human Lymph Node Samples

Dagmar Rimek,1* Sachin Tyagi,2 and Reinhard Kappe3

Department of Medical Microbiology and Hospital Hygiene, University Hospital, D-18057 Rostock,1 Institute of Medical Microbiology and Hygiene, D-99089 Erfurt, Germany,3 Department of Microbiology, Subharati Medical College, Meerut, India2

Received 10 December 2001/ Returned for modification 19 March 2002/ Accepted 18 May 2002

We compared the performance of two PCR assays, an IS6110-based in-house protocol and the COBAS AMPLICOR MTB PCR (COBAS MTB) system, for the detection of Mycobacterium tuberculosis complex in 43 human lymph node samples from 40 patients. For the in-house PCR and the COBAS MTB assays, respectively, sensitivities were 87.5% versus 45.5% (P < 0.05), specificities were 100.0% versus 91.3% (P > 0.05), and inhibition rates were 4.8% versus 19.5% (P < 0.05). For the COBAS MTB system, additional N-acetyl-L-cysteine-NaOH pretreatment of the samples changed neither the inhibition rate nor the sensitivity significantly.

* Corresponding author. Mailing address: Department of Medical Microbiology and Hospital Hygiene, University Hospital, Schillingallee 70, D-18057 Rostock, Germany. Phone: 49-381-4945943. Fax: 49-381-4945902. E-mail: dagmar.rimek{at}med.uni-rostock.de.

Journal of Clinical Microbiology, August 2002, p. 3089-3092, Vol. 40, No. 8
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.8.3089-3092.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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