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Journal of Clinical Microbiology, September 2002, p. 3172-3178, Vol. 40, No. 9
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.9.3172-3178.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

groESL Sequence Determination, Phylogenetic Analysis, and Species Differentiation for Viridans Group Streptococci

Lee-Jene Teng,1,2* Po-Ren Hsueh,2 Jui-Chang Tsai,3,4 Pin-Wun Chen,1 Jia-Chuan Hsu,1 Hsin-Chih Lai,1,2 Chun-Nan Lee,1,2 and Shen-Wu Ho1,2

School of Medical Technology,1 Department of Neurosurgery,3 Center for Optoelectronic Biomedicine, National Taiwan University College of Medicine,4 Department of Laboratory Medicine, National Taiwan University Hospital, Taipei, Taiwan2

Received 28 February 2002/ Returned for modification 20 April 2002/ Accepted 9 June 2002

The full-length sequences of the groESL genes (also known as cpn10/60) of Streptococcus anginosus, Streptococcus constellatus, Streptococcus gordonii, and Streptococcus sanguis and the near full-length sequence of the groESL genes of Streptococcus intermedius, Streptococcus bovis, Streptococcus mitis, Streptococcus mutans, Streptococcus oralis, and Streptococcus salivarius were determined. The lengths of the groES genes from the 10 species listed above ranged from 282 to 288 bp, and the full-length sequences of groEL determined for 4 species (S. anginosus, S. constellatus, S. gordonii, and S. sanguis) revealed that each was 1,623 bp. The intergenic region (spacer) between the groES and groEL genes varies in size (15 to 111 bp) and sequence between species. The variation of the groES sequences among the species tested was greater (62.1 to 95.1% nucleotide sequence identities) than that of the groEL sequences (77.2 to 95.2% nucleotide sequence identities). Phylogenetic analysis of the groES and groEL genes yielded evolutionary trees similar to the tree constructed by use of the 16S rRNA gene. The intraspecies variation of the spacer was minimal for clinical isolates of some species. The groESL sequence data provide an additional parameter for identification of viridans group streptococcal species.

* Corresponding author. Mailing address: School of Medical Technology, National Taiwan University College of Medicine, No. 1, Chang-Te Street, Taipei 100, Taiwan. Phone: 886-2-23123456, ext. 6918. Fax: 886-2-23711574. E-mail: ljteng{at}ha.mc.ntu.edu.tw.

Journal of Clinical Microbiology, September 2002, p. 3172-3178, Vol. 40, No. 9
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.9.3172-3178.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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