This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miko, A.
Right arrow Articles by Helmuth, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miko, A.
Right arrow Articles by Helmuth, R.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, September 2002, p. 3184-3191, Vol. 40, No. 9
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.9.3184-3191.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Molecular Characterization of Multiresistant d-Tartrate-Positive Salmonella enterica Serovar Paratyphi B Isolates

Angelika Miko, Beatriz Guerra, Andreas Schroeter, Christina Dorn, and Reiner Helmuth*

National Salmonella Reference Laboratory, Federal Institute for Health Protection of Consumers and Veterinary Medicine, 12277 Berlin, Germany

Received 18 December 2001/ Returned for modification 7 April 2001/ Accepted 20 June 2002

Since 1996, the National Salmonella Reference Laboratory of Germany has received an increasing number of Salmonella enterica subsp. enterica serovar Paratyphi B isolates. Nearly all of these belonged to the dextrorotatory tartrate-positive variant (S. enterica subsp. enterica serovar Paratyphi B dT+), formerly called S. enterica subsp. enterica serovar Java. A total of 55 selected contemporary and older S. enterica subsp. enterica serovar Paratyphi B dT+ isolates were analyzed by plasmid profiling, antimicrobial resistance testing, pulsed-field gel electrophoresis, IS200 profiling, and PCR-based detection of integrons. The results showed a high genetic heterogeneity among 10 old strains obtained from 1960 to 1993. In the following years, however, new distinct multiresistant S. enterica subsp. enterica serovar Paratyphi B dT+ clones emerged, and one clonal lineage successfully displaced the older ones. Since 1994, 88% of the isolates investigated were multiple drug resistant. Today, a particular clone predominates in some German poultry production lines, poultry products, and various other sources. It was also detected in contemporary isolates from two neighboring countries as well.

* Corresponding author. Mailing address: Federal Institute for Health Protection of Consumers and Veterinary Medicine (BgVV), Diedersdorfer Weg 1, 12277 Berlin, Germany. Phone: 49 30 8412 2233. Fax: 49 30 8412 2953. E-mail: r.helmuth{at}bgvv.de.

Journal of Clinical Microbiology, September 2002, p. 3184-3191, Vol. 40, No. 9
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.9.3184-3191.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • van Asselt, E. D., Thissen, J. T. N. M., van der Fels-Klerx, H. J. (2009). Salmonella serotype distribution in the Dutch broiler supply chain. Poult. Sci. 88: 2695-2701 [Abstract] [Full Text]  
  • Chia, T. W. R., Fegan, N., Dykes, G. A., Huehn, S., Bunge, C., Junker, E., Helmuth, R., Malorny, B. (2009). Potential Role for the 4,12 Antigen in the Prevalence of Clonal Salmonella Serovars in Poultry. Appl. Environ. Microbiol. 75: 3377-3378 [Full Text]  
  • Huehn, S., Bunge, C., Junker, E., Helmuth, R., Malorny, B. (2009). Poultry-Associated Salmonella enterica subsp. enterica Serovar 4,12:d:- Reveals High Clonality and a Distinct Pathogenicity Gene Repertoire. Appl. Environ. Microbiol. 75: 1011-1020 [Abstract] [Full Text]  
  • Han, K. H., Choi, S. Y., Lee, J. H., Lee, H., Shin, E. H., Agtini, M. D., von Seidlein, L., Ochiai, R. L., Clemens, J. D., Wain, J., Hahn, J.-S., Lee, B. K., Song, M., Chun, J., Kim, D. W. (2006). Isolation of Salmonella enterica subspecies enterica serovar Paratyphi B dT+, or Salmonella Java, from Indonesia and alteration of the d-tartrate fermentation phenotype by disrupting the ORF STM 3356.. J Med Microbiol 55: 1661-1665 [Abstract] [Full Text]  
  • Kim, S., Frye, J. G., Hu, J., Fedorka-Cray, P. J., Gautom, R., Boyle, D. S. (2006). Multiplex PCR-Based Method for Identification of Common Clinical Serotypes of Salmonella enterica subsp. enterica. J. Clin. Microbiol. 44: 3608-3615 [Abstract] [Full Text]  
  • Tracz, D. M., Tabor, H., Jerome, M., Ng, L.-K., Gilmour, M. W. (2006). Genetic Determinants and Polymorphisms Specific for Human-Adapted Serovars of Salmonella enterica That Cause Enteric Fever.. J. Clin. Microbiol. 44: 2007-2018 [Abstract] [Full Text]  
  • Miko, A., Pries, K., Schroeter, A., Helmuth, R. (2005). Molecular mechanisms of resistance in multidrug-resistant serovars of Salmonella enterica isolated from foods in Germany. J Antimicrob Chemother 56: 1025-1033 [Abstract] [Full Text]  
  • Weill, F.-X., Fabre, L., Grandry, B., Grimont, P. A. D., Casin, I. (2005). Multiple-Antibiotic Resistance in Salmonella enterica Serotype Paratyphi B Isolates Collected in France between 2000 and 2003 Is Due Mainly to Strains Harboring Salmonella Genomic Islands 1, 1-B, and 1-C. Antimicrob. Agents Chemother. 49: 2793-2801 [Abstract] [Full Text]  
  • Romero, L., Lopez, L., Martinez-Martinez, L., Guerra, B., Hernandez, J. R., Pascual, A. (2004). Characterization of the first CTX-M-14-producing Salmonella enterica serotype Enteritidis isolate. J Antimicrob Chemother 53: 1113-1114 [Full Text]  
  • Miko, A., Pries, K., Schroeter, A., Helmuth, R. (2003). Multiple-Drug Resistance in D-Tartrate-Positive Salmonella enterica Serovar Paratyphi B Isolates from Poultry Is Mediated by Class 2 Integrons Inserted into the Bacterial Chromosome. Antimicrob. Agents Chemother. 47: 3640-3643 [Abstract] [Full Text]  
  • Prager, R., Rabsch, W., Streckel, W., Voigt, W., Tietze, E., Tschape, H. (2003). Molecular Properties of Salmonella enterica Serotype Paratyphi B Distinguish between Its Systemic and Its Enteric Pathovars. J. Clin. Microbiol. 41: 4270-4278 [Abstract] [Full Text]  
  • Malorny, B., Bunge, C., Helmuth, R. (2003). Discrimination of d-Tartrate-Fermenting and -Nonfermenting Salmonella enterica subsp. enterica Isolates by Genotypic and Phenotypic Methods. J. Clin. Microbiol. 41: 4292-4297 [Abstract] [Full Text]  
  • van Duijkeren, E., Wannet, W. J. B., Houwers, D. J., van Pelt, W. (2003). Antimicrobial Susceptibilities of Salmonella Strains Isolated from Humans, Cattle, Pigs, and Chickens in The Netherlands from 1984 to 2001. J. Clin. Microbiol. 41: 3574-3578 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»