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Journal of Clinical Microbiology, September 2002, p. 3277-3280, Vol. 40, No. 9
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.9.3277-3280.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Detection of Trichomonas vaginalis on Modified Columbia Agar in the Routine Laboratory

Angelika Stary,* Angelika Kuchinka-Koch, and Lilianna Teodorowicz

Outpatients' Center for Diagnosis of Infectious Venerodermatological Diseases, A-1210 Vienna, Austria

Received 4 February 2002/ Returned for modification 14 April 2002/ Accepted 23 June 2002

Broth culture of Trichomonas vaginalis is considered the "gold standard" for the diagnosis of trichomoniasis. Two studies were carried out to evaluate modified Columbia agar (MCA) for the isolation of T. vaginalis from clinical samples. Study I compared isolation on MCA to that on liquid medium with 889 vaginal samples. Out of 63 samples positive for T. vaginalis (7.1% of total), MCA identified 62 (98.4%) and broth identified 58 (92.1%). In study II, trichomoniasis was diagnosed within the scope of a screening program for a total of 39,585 men and women by culture on MCA and direct microscopy. Culture on MCA detected 199 (98.5%) and Gram staining detected 163 (80.7%) of 202 positive specimens. Wet-mount preparations used for symptomatic patients identified 103 (92.8%) of 111 cases. Culture of T. vaginalis from clinical samples on MCA is highly sensitive and reliable, as well as timesaving, and therefore suitable for screening of symptomatic and asymptomatic individuals.

* Corresponding author. Mailing address: Outpatients' Center for Diagnosis of Infectious Venerodermatological Diseases, Franz-Jonas-Platz 8/2/3, A-1210 Vienna, Austria. Phone: 43-1-2707660. Fax: 43-1-27076609. E-mail: angelika.stary{at}univie.ac.at.

Journal of Clinical Microbiology, September 2002, p. 3277-3280, Vol. 40, No. 9
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.9.3277-3280.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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Copyright © 2002 by the American Society for Microbiology. All rights reserved.