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Journal of Clinical Microbiology, September 2002, p. 3346-3349, Vol. 40, No. 9
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.9.3346-3349.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Leptotrichia amnionii sp. nov., a Novel Bacterium Isolated from the Amniotic Fluid of a Woman after Intrauterine Fetal Demise

Sanjay K. Shukla,1* Paul R. Meier,2 Paul D. Mitchell,3 Daniel N. Frank,4 and Kurt D. Reed1

Clinical Research Center, Marshfield Medical Research and Education Foundation,1 Department of Obstetrics and Gynecology, Marshfield Clinic,2 Marshfield Laboratories, Marshfield, Wisconsin 54449,3 Department of Molecular, Cellular, and Developmental Biology, The University of Colorado, Boulder, Colorado 803094

Received 18 March 2002/ Returned for modification 21 June 2002/ Accepted 30 June 2002

A novel bacterium was isolated and characterized from the amniotic fluid of a woman who experienced intrauterine fetal demise in the second trimester of pregnancy. The bacterium was a slow-growing, gram-negative anaerobic coccobacillus belonging to the genus Leptotrichia. Unlike Leptotrichia sanguinegens, the isolate did not grow in chopped-meat glucose broth or on sheep blood agar upon subculturing. The isolate was characterized by sequencing and analyzing its 16S rRNA gene. The 1,493-bp 16S ribosomal DNA sequence had only 96% homology with L. sanguinegens. Several phylogenetic analyses indicated that L. amnionii is a distinct species and most closely related to L. sanguiegens.

* Corresponding author. Mailing address: Clinical Research Center, Marshfield Medical Research and Education Foundation, 1000 North Oak Avenue, Marshfield, WI 54449. Phone: (715) 389-5363. Fax: (715) 389-3808. E-mail: shuklas{at}mmrf.mfldclin.edu.

Journal of Clinical Microbiology, September 2002, p. 3346-3349, Vol. 40, No. 9
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.9.3346-3349.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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