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Journal of Clinical Microbiology, September 2002, p. 3485-3488, Vol. 40, No. 9
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.9.3485-3488.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Direct PCR Detection of Burkholderia cepacia Complex and Identification of Its Genomovars by Using Sputum as Source of DNA

Pavel Devínek,^1* Hana Hrbáková,² Ondej Cinek,¹ Jana Bartoová,¹ Otakar Ny,² Alexandr Nemec,³ and Petr Pohunek¹

2nd Department of Pediatrics, 2nd Medical School of Charles University,¹ Department of Medical Microbiology, Motol University Hospital,² National Institute of Public Health, Prague, Czech Republic³

Received 15 April 2002/ Returned for modification 16 May 2002/ Accepted 23 June 2002

We developed a nested PCR assay that detects the recA gene of the Burkholderia cepacia complex in sputum. The product of the first PCR round is also used to identify the genomovar of the pathogen. The protocol achieves high sensitivity and specificity with simple interpretation of genomovar status.

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